Publication detail

Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots

KREJČOVÁ, L. NEJDL, L. HYNEK, D. KŘÍŽKOVÁ, S. KOPEL, P. ADAM, V. KIZEK, R.

Original Title

Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots

Type

journal article in Web of Science

Language

English

Original Abstract

In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an electrochemical label as quantum dots (QDs). For detection of the metal and protein part of the resulting HA-CdTe complex, two differential pulse voltammetric methods were used. The procedure includes automated robotic isolation and electrochemical analysis of the isolated product. The isolation procedure was based on the binding of paramagnetic particles (MPs) with glycan (Gly), where glycan was used as the specific receptor for linkage of the QD-labeled hemagglutinin.

Keywords

influenza hemagglutinin; electrochemical detection; paramagnetic particles; glycan; quantum dot labeling

Authors

KREJČOVÁ, L.; NEJDL, L.; HYNEK, D.; KŘÍŽKOVÁ, S.; KOPEL, P.; ADAM, V.; KIZEK, R.

RIV year

2013

Released

13. 12. 2013

Publisher

MDPI

ISBN

1420-3049

Periodical

MOLECULES

Year of study

18

Number

12

State

Swiss Confederation

Pages from

15573

Pages to

15586

Pages count

14

URL

https://www.mdpi.com/1420-3049/18/12/15573

Full text in the Digital Library

http://hdl.handle.net/11012/180499

BibTex

@article{BUT103909,
  author="Ludmila {Krejčová} and Lukáš {Nejdl} and David {Hynek} and Soňa {Křížková} and Pavel {Kopel} and Vojtěch {Adam} and René {Kizek}",
  title="Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots",
  journal="MOLECULES",
  year="2013",
  volume="18",
  number="12",
  pages="15573--15586",
  doi="10.3390/molecules181215573",
  issn="1420-3049",
  url="https://www.mdpi.com/1420-3049/18/12/15573"
}