Publication detail

Use of brightness wavelet transformation for automated analysis of serum metallothioneins- and zinc-containing proteins by Western blots to subclassify childhood solid tumours

VYSLOUŽILOVÁ, L. KŘÍŽKOVÁ, S. ANÝZ, J. HYNEK, D. HRABĚTA, J. KRUSEOVÁ, J. ECKSCHLAGER, T. ADAM, V. ŠTĚPÁNKOVÁ, O. KIZEK, R.

Original Title

Use of brightness wavelet transformation for automated analysis of serum metallothioneins- and zinc-containing proteins by Western blots to subclassify childhood solid tumours

Type

journal article in Web of Science

Language

English

Original Abstract

In this study, we determined serum levels of metallothioneins (MTs) and zinc in children with solid tumours (neuroblastoma, Hodgkin lymphoma, medulloblastoma, osteosarcoma, Ewing sarcoma and nephroblastoma) by differential pulse voltammetry Brdicka reaction and ELISA. Zn(II) level in patients sera was 40% compared to controls, contrariwise, MT level was 4.2 higher in patients. No significant differences among single diagnoses were found both for Zn(II) and MT. When determined Zn(II)/MT ratio, in controls its value was 24.6, but it was 2.6 in patients. After Western-blotting with anti-MT and anti-Zn chicken antibodies, variable intensities of the bands within the samples were observed. The brightness curve obtained for each sample both for MT- and Zn blots was further analysed to produce a list of band positions together with some complementary information related to the intensity of the observed bands by the optimised algorithm.

Keywords

wavelet transformation, metallothionein, Western blot

Authors

VYSLOUŽILOVÁ, L.; KŘÍŽKOVÁ, S.; ANÝZ, J.; HYNEK, D.; HRABĚTA, J.; KRUSEOVÁ, J.; ECKSCHLAGER, T.; ADAM, V.; ŠTĚPÁNKOVÁ, O.; KIZEK, R.

RIV year

2013

Released

1. 6. 2013

ISBN

0173-0835

Periodical

Electrophoresis

Year of study

34

Number

11

State

Federal Republic of Germany

Pages from

1637

Pages to

1648

Pages count

12

BibTex

@article{BUT101161,
  author="Lenka {Vysloužilová} and Soňa {Křížková} and Jiří {Anýz} and David {Hynek} and Jan {Hraběta} and Jarmila {Kruseová} and Tomáš {Eckschlager} and Vojtěch {Adam} and Olga {Štěpánková} and René {Kizek}",
  title="Use of brightness wavelet transformation for automated analysis of serum metallothioneins- and zinc-containing proteins by Western blots to subclassify childhood solid tumours",
  journal="Electrophoresis",
  year="2013",
  volume="34",
  number="11",
  pages="1637--1648",
  doi="10.1002/elps.201200561",
  issn="0173-0835"
}