Publication detail

Development of Simple Splitless Gradient Liquid Chromatographic system

ŠESTÁK, J. a kol.

Original Title

Development of Simple Splitless Gradient Liquid Chromatographic system

Type

conference paper

Language

English

Original Abstract

A simple gradient liquid chromatographic system for micro and nano column separations has been assembled and tested. The device involves simple splitless gradient generation and sample delivery. Experimental configuration consists of an OEM programmable syringe pump equipped with a glass microsyringe and ten-port selector valve. Automated system was controlled via LabVIEW development environment. Continuous gradients of mobile phase were created in the syringe barrel by successive sucking of adequate volumes of four mobile phases with gradually decreasing acetonitrile concentration and due to turbulent mixing occurred at the syringe needle-barrel boundary. System performance was evaluated using test mixture of six alkylphenones. Good values of relative standard deviation of retention times were received (intra-day RSD < 0.3 %, inter-day RSD < 1.4 %). Repeatability of sample injection in the range 0.1 – 3 ul was evaluated. The peak areas were measured and statistically evaluated (RSD < 5 %).

Keywords

splitless gradient, nano column, alkylphenones

Authors

ŠESTÁK, J. a kol.

RIV year

2012

Released

7. 12. 2012

Publisher

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

ISBN

978-80-214-4644-1

Book

Studentská odborná konference Chemie je život - Sborník příspěvků

Pages from

446

Pages to

449

Pages count

4

BibTex

@inproceedings{BUT97457,
  author="Jozef {Šesták}",
  title="Development of Simple Splitless Gradient Liquid Chromatographic system",
  booktitle="Studentská odborná konference Chemie je život - Sborník příspěvků",
  year="2012",
  pages="446--449",
  publisher="Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno",
  isbn="978-80-214-4644-1"
}