Publication detail

Accumulation of Cadmium by Transgenic Tobacco Plants (Nicotiana tabacum L.) Carrying Yeast Metallothionein Gene Revealed by Electrochemistry

KRYŠTOFOVÁ, O. ZÍTKA, O. KŘÍŽKOVÁ, S. HYNEK, D. SHESTIVSKA, V. ADAM, V. HUBÁLEK, J. MACKOVÁ, M. MACEK, T. ZEHNÁLEK, J. BABULA, P. HAVEL, L. KIZEK, R.

Original Title

Accumulation of Cadmium by Transgenic Tobacco Plants (Nicotiana tabacum L.) Carrying Yeast Metallothionein Gene Revealed by Electrochemistry

Type

journal article in Web of Science

Language

English

Original Abstract

Phytoremediation of heavy metals includes phytoextraction and phytoaccumulation, i.e. accumulation of pollution from soils or water into the plants. Inorganic pollutants like heavy metals cannot be chemically degraded and thus it has to be accumulated in roots and leaves of the plants in non-toxic forms. In this study, the influence of cadmium on transgenic Nicotiana tabacum plants carrying yeast metallothionein was investigated. Tobacco plants were exposed to 0, 50, 100 and 250 uM CdCl2 for 96 hours. Growth parameters as well as changes in content of cysteine, oxidized/reduced glutathione, phytochelatins (PC2, PC3 and PC4) were monitored using high performance liquid chromatography with electrochemical detection. Moreover, we exploited differential pulse voltammetry Brdicka reaction for determination of metallothionein and verified the results obtained by dot blot assay. In addition, all results were compared with results obtained using non-transgenic tobacco plants. Our results indicate protective role of metallothionein as well as its role in cadmium accumulation in roots of transgenic plants.

Keywords

Metallothionein, Tobacco, Nicotiana tabacum, Thiols, Phytoremediation, Electrochemical detection, Brdicka reaction, Voltametry, Liquid chromatography, Heavy metal

Authors

KRYŠTOFOVÁ, O.; ZÍTKA, O.; KŘÍŽKOVÁ, S.; HYNEK, D.; SHESTIVSKA, V.; ADAM, V.; HUBÁLEK, J.; MACKOVÁ, M.; MACEK, T.; ZEHNÁLEK, J.; BABULA, P.; HAVEL, L.; KIZEK, R.

RIV year

2012

Released

1. 2. 2012

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

7

Number

2

State

Republic of Serbia

Pages from

886

Pages to

907

Pages count

22

BibTex

@article{BUT92983,
  author="Olga {Kryštofová} and Ondřej {Zítka} and Soňa {Křížková} and David {Hynek} and Violetta {Shestivska} and Vojtěch {Adam} and Jaromír {Hubálek} and Martina {Macková} and Tomáš {Macek} and Josef {Zehnálek} and Petr {Babula} and Ladislav {Havel} and René {Kizek}",
  title="Accumulation of Cadmium by Transgenic Tobacco Plants (Nicotiana tabacum L.) Carrying Yeast Metallothionein Gene Revealed by Electrochemistry",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2012",
  volume="7",
  number="2",
  pages="886--907",
  issn="1452-3981"
}