Publication detail

Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver

TRNKOVÁ, L. KŘÍŽKOVÁ, S. ADAM, V. HUBÁLEK, J. KIZEK, R.

Original Title

Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver

English Title

Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver

Type

journal article - other

Language

en

Original Abstract

In this paper, heavy metal biosensor based on immobilization of metallothionein (MT) to the surface of carbon paste electrode (CPE) via anti-MT-antibodies is reported. First, the evaluation of MT electroactivity was done. The attention was focused on the capturing of MT to the CPE surface. Antibodies incorporated and mixed into carbon paste were stable; even after two weeks the observed changes in signal height were lower than 5%. Further, the interaction of MT with polyclonal chicken antibodies incorporated in carbon paste electrode was determined by square-wave voltammetry. In the voltammogram, two signals - labelled as cys(MT) and W(a) - were observed. The cyst,AT corresponded to -SH moieties of MT and W(a) corresponded to tryptophan residues of chicken antibodies. Time of interaction (300 s) and MT concentration (125 mu g/ml) were optimized to suggest a silver(I) ions biosensor. Biosensor (CPE modified with anti-MT antibody) prepared under the optimized conditions was then used for silver(I) ions detection. The detection limit (3 S/N) for silver(I) ions was estimated as 0.5 nM. The proposed biosensor was tested by detection spiking of silver(I) ions in various water samples (from very pure distilled water to rainwater). Recoveries varied from 74 to 104%.

English abstract

In this paper, heavy metal biosensor based on immobilization of metallothionein (MT) to the surface of carbon paste electrode (CPE) via anti-MT-antibodies is reported. First, the evaluation of MT electroactivity was done. The attention was focused on the capturing of MT to the CPE surface. Antibodies incorporated and mixed into carbon paste were stable; even after two weeks the observed changes in signal height were lower than 5%. Further, the interaction of MT with polyclonal chicken antibodies incorporated in carbon paste electrode was determined by square-wave voltammetry. In the voltammogram, two signals - labelled as cys(MT) and W(a) - were observed. The cyst,AT corresponded to -SH moieties of MT and W(a) corresponded to tryptophan residues of chicken antibodies. Time of interaction (300 s) and MT concentration (125 mu g/ml) were optimized to suggest a silver(I) ions biosensor. Biosensor (CPE modified with anti-MT antibody) prepared under the optimized conditions was then used for silver(I) ions detection. The detection limit (3 S/N) for silver(I) ions was estimated as 0.5 nM. The proposed biosensor was tested by detection spiking of silver(I) ions in various water samples (from very pure distilled water to rainwater). Recoveries varied from 74 to 104%.

Keywords

Heavy metal; Silver; Metallothionein; Square wave voltammetry; Water; Environmental analysis

RIV year

2011

Released

15.01.2011

Pages from

2201

Pages to

2207

Pages count

7

BibTex


@article{BUT88376,
  author="Libuše {Trnková} and Soňa {Křížková} and Vojtěch {Adam} and Jaromír {Hubálek} and René {Kizek}",
  title="Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver",
  annote="In this paper, heavy metal biosensor based on immobilization of metallothionein (MT) to the surface of carbon paste electrode (CPE) via anti-MT-antibodies is reported. First, the evaluation of MT electroactivity was done. The attention was focused on the capturing of MT to the CPE surface. Antibodies incorporated and mixed into carbon paste were stable; even after two weeks the observed changes in signal height were lower than 5%. Further, the interaction of MT with polyclonal chicken antibodies incorporated in carbon paste electrode was determined by square-wave voltammetry. In the voltammogram, two signals - labelled as cys(MT) and W(a) - were observed. The cyst,AT corresponded to -SH moieties of MT and W(a) corresponded to tryptophan residues of chicken antibodies. Time of interaction (300 s) and MT concentration (125 mu g/ml) were optimized to suggest a silver(I) ions biosensor. Biosensor (CPE modified with anti-MT antibody) prepared under the optimized conditions was then used for silver(I) ions detection. The detection limit (3 S/N) for silver(I) ions was estimated as 0.5 nM. The proposed biosensor was tested by detection spiking of silver(I) ions in various water samples (from very pure distilled water to rainwater). Recoveries varied from 74 to 104%.",
  chapter="88376",
  number="5",
  volume="2011 (26)",
  year="2011",
  month="january",
  pages="2201--2207",
  type="journal article - other"
}