Publication detail

Biotin-modified glutathione as functionalized coating for bioconjugation of CdTe-based quantum dots

VACULOVIČOVÁ, M. PEKÁRKOVÁ, J. JANŮ, L. DRBOHLAVOVÁ, J. ADAM, V. HUBÁLEK, J. KIZEK, R.

Original Title

Biotin-modified glutathione as functionalized coating for bioconjugation of CdTe-based quantum dots

Type

journal article in Web of Science

Language

English

Original Abstract

In this study, biotin-conjugated glutathione was synthesized using peptide bonding of the biotin carboxy group and amino group of the g-glutamic acid to prepare an alternative coating for CdTe quantum dots (QDs). This type of coating combines the functionality of the biotin with the fluorescent properties of the QDs to create a specific, high-affinity fluorescent probe able to react with avidin, streptavidin and/or neutravidin. Biotin-functionalized glutathione-coated CdTe QDs were prepared by a simple one-step method using Na(2)TeO(3) and CdCl(2). Obtained QDs were separated from the excess of the biotin-conjugated glutathione by CE employing 300mM borate buffer with pH 7.8 as a background electrolyte. The detection of sample components was performed by the photometric detection at 214 nm and LIF employing Ar(+) ion laser (488 nm).

Keywords

Biotin-conjugated glutathione, CE, Glutathione, Quantum dot, Streptavidin– biotin

Authors

VACULOVIČOVÁ, M.; PEKÁRKOVÁ, J.; JANŮ, L.; DRBOHLAVOVÁ, J.; ADAM, V.; HUBÁLEK, J.; KIZEK, R.

RIV year

2011

Released

24. 5. 2011

Publisher

Wiley

ISBN

0173-0835

Periodical

Electrophoresis

Year of study

32

Number

13

State

Federal Republic of Germany

Pages from

1619

Pages to

1622

Pages count

4

BibTex

@article{BUT75320,
  author="Markéta {Vaculovičová} and Jana {Pekárková} and Libor {Janů} and Jana {Drbohlavová} and Vojtěch {Adam} and Jaromír {Hubálek} and René {Kizek}",
  title="Biotin-modified glutathione as functionalized coating for bioconjugation of CdTe-based quantum dots",
  journal="Electrophoresis",
  year="2011",
  volume="32",
  number="13",
  pages="1619--1622",
  issn="0173-0835"
}