Publication detail

Simple Measurement of Acetylcholinestrase Activity

ONDRUCH, V., KREJČÍ, J., KREJČOVÁ, D., VESELÁ, R.

Original Title

Simple Measurement of Acetylcholinestrase Activity

English Title

Simple Measurement of Acetylcholinestrase Activity

Type

conference proceedings

Language

en

Original Abstract

In this work are presented electrochemical measurements which consists of several steps. They are aimed to simplify enzyme activity observations. First of all the background current correction is made. The calibration of the current response follows. It is made by enzyme reaction product addition. From the determined current (numerically computed) and from the known concentration the calibration constant for the current dependence on the concentration is calculated. In the next step the enzyme solution is added to the reaction vessel. The current increase in dependence on arising product concentration in time is monitored. The concentration is computed using the known calibration constant. The device enables both the concentration and its actual changes. In the module of evaluation is from the measured data determined the enzyme activity. The method was tested using Acetylcholinesterase enzyme.

English abstract

In this work are presented electrochemical measurements which consists of several steps. They are aimed to simplify enzyme activity observations. First of all the background current correction is made. The calibration of the current response follows. It is made by enzyme reaction product addition. From the determined current (numerically computed) and from the known concentration the calibration constant for the current dependence on the concentration is calculated. In the next step the enzyme solution is added to the reaction vessel. The current increase in dependence on arising product concentration in time is monitored. The concentration is computed using the known calibration constant. The device enables both the concentration and its actual changes. In the module of evaluation is from the measured data determined the enzyme activity. The method was tested using Acetylcholinesterase enzyme.

Keywords

Electrochemical sensor, enzyme activity

Released

19.01.2005

Publisher

Faculty of Electric Engineering, University Teknologi MARA

Location

Kuala Lumpur, Malaisia

ISBN

0-7803-9371-6

Book

AsiaSense 2005

Pages from

121

Pages to

124

Pages count

4

BibTex


@proceedings{BUT64196,
  author="Vít {Ondruch} and Jan {Krejčí}",
  title="Simple Measurement of Acetylcholinestrase Activity",
  annote="In this work are presented electrochemical measurements which consists of several steps. They are aimed to simplify enzyme activity observations.
First of all the background current correction is made.  The calibration of the current response follows. It is made by enzyme reaction product addition. From the determined current (numerically computed) and from the known concentration the calibration constant for the current dependence on the concentration is calculated. In the next step the enzyme solution is added to the reaction vessel. The current increase in dependence on arising product concentration in time is monitored. The concentration is computed using the known  calibration constant. The device enables both the concentration and its actual changes. In the module of evaluation is from the measured data determined the enzyme activity.
The method was tested using Acetylcholinesterase enzyme.",
  address="Faculty of Electric Engineering, University Teknologi MARA",
  booktitle="AsiaSense 2005",
  chapter="64196",
  institution="Faculty of Electric Engineering, University Teknologi MARA",
  year="2005",
  month="january",
  pages="121",
  publisher="Faculty of Electric Engineering, University Teknologi MARA",
  type="conference proceedings"
}