Publication detail

Rapid and Ultrasensitive Method for Determination of Phytochelatin2 using High Performance Liquid Chromatography with Electrochemical Detection

ZÍTKA, O. ŠKUTKOVÁ, H. KRYŠTOFOVÁ, O. ŠOBROVÁ, P. ADAM, V. ZEHNÁLEK, J. BEKLOVÁ, M. HUBÁLEK, J. PROVAZNÍK, I. KIZEK, R.

Original Title

Rapid and Ultrasensitive Method for Determination of Phytochelatin2 using High Performance Liquid Chromatography with Electrochemical Detection

Type

journal article in Web of Science

Language

English

Original Abstract

Plants respond to heavy metal toxicity in a variety of different ways including synthesis of phytochelatins. The synthesis of phytochelatins is catalyzed by gama-Glu-Cys dipeptidyl transpeptidase named as phytochelatin synthase (PCS). The main aim of this study was to optimize high performance liquid chromatography coupled with electrochemical detector for determination of phytochelatin2. The optimized procedure was subsequently used for determination of the mentioned molecules with special attention aimed at the possibility to determine PC2 after activation of PCS in the tobacco BY-2 cells treated with different concentrations of cadmium(II) ions.

Keywords

cadmium, glutathione, phytochelatin, high performance liquid chromatography, electrochemical detector, tobacco BY-2 cell suspension

Authors

ZÍTKA, O.; ŠKUTKOVÁ, H.; KRYŠTOFOVÁ, O.; ŠOBROVÁ, P.; ADAM, V.; ZEHNÁLEK, J.; BEKLOVÁ, M.; HUBÁLEK, J.; PROVAZNÍK, I.; KIZEK, R.

RIV year

2011

Released

1. 5. 2011

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

2011 (6)

Number

5

State

Republic of Serbia

Pages from

1367

Pages to

1381

Pages count

15

BibTex

@article{BUT50164,
  author="Ondřej {Zítka} and Helena {Vítková} and Olga {Kryštofová} and Pavlína {Adam} and Vojtěch {Adam} and Josef {Zehnálek} and Miroslava {Beklová} and Jaromír {Hubálek} and Valentine {Provazník} and René {Kizek}",
  title="Rapid and Ultrasensitive Method for Determination of Phytochelatin2 using High Performance Liquid Chromatography with Electrochemical Detection",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2011",
  volume="2011 (6)",
  number="5",
  pages="1367--1381",
  issn="1452-3981"
}