Publication detail

Proving Tumour Cells by Acute Nutritional/Energy Deprivation as a Survival Threat: A Task for Microscopy

JANEČKOVÁ, H. VESELÝ, P. CHMELÍK, R.

Original Title

Proving Tumour Cells by Acute Nutritional/Energy Deprivation as a Survival Threat: A Task for Microscopy

Type

journal article in Web of Science

Language

English

Original Abstract

Malignant cells appear to possess a special aptitude for survival. We attempted to prove this in vitro by an acute nutritional and energy deprivation as a survival threat. A phosphate-buffered saline (PBS) survival test in cell culture allowed static observations. These were supplemented by classic and quantitative phase-contrast time-lapse microscopy. From one normal and four neoplastic cell populations, no cells survived 77 hours exposure to PBS. Only G3S2 derived from a human breast carcinoma survived 60 hours. Cells in sparse culture were more vulnerable than those in dense. Epithelial cells were more vigorous than mesenchymal cells. Cells of greater malignancy resisted longer. Evaluation in culture as detailed by digital holographic microscopy (DHM) revealed an increase in the compactness of the intracellular mass motility from normal to metastasizing mesenchymal cells, thus reaching the level of epithelial G3S2 cells. Studying the PBS survival test with DHM opens a new approach to investigations of the structural integrity of neoplastic cells.

Keywords

Tumour cell, nutritional deprivation, energy deprivation, cell survival, cell death, digital holographic microscopy, dynamic phase difference

Authors

JANEČKOVÁ, H.; VESELÝ, P.; CHMELÍK, R.

RIV year

2009

Released

6. 6. 2009

Publisher

International Institute of Anticancer Research

Location

Greece

ISBN

0250-7005

Periodical

ANTICANCER RESEARCH

Year of study

29

Number

6

State

Hellenic Republic

Pages from

2339

Pages to

2345

Pages count

7

URL

http://www.iiar-anticancer.org/

BibTex

@article{BUT49714,
  author="Hana {Uhlířová} and Pavel {Veselý} and Radim {Chmelík}",
  title="Proving Tumour Cells by Acute Nutritional/Energy Deprivation as a Survival Threat: A Task for Microscopy",
  journal="ANTICANCER RESEARCH",
  year="2009",
  volume="29",
  number="6",
  pages="2339--2345",
  issn="0250-7005",
  url="http://www.iiar-anticancer.org/"
}