Publication detail

Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance

HÚSKA, D. FABRIK, I. BALOUN, J. ADAM, V. MASAŘÍK, M. HUBÁLEK, J. VAŠKŮ, A. TRNKOVÁ, L. HORNA, A. ZEMAN, L. KIZEK, R.

Original Title

Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance

Type

journal article - other

Language

English

Original Abstract

Treatment strategies for tumour diseases are progressively focusing on personalization of medicine. However, this focus requires methods revealing the early general biological mechanisms, including the formation anti-cancer drugs' resistance. The low molecular mass protein metallothionein is thought to be the crucial for the formation of resistance in tumour treatment based on the platinum-cytostatics. The interactions between metallothionein (MT) and cisplatin were determined by the adsorptive transfer stripping technique coupled with the differential pulse votlammetry Brdicka's reaction. The signals related to the MT-cisplatin complex appeared at -0.9 V. The formation of this complex depended on the time of interaction between cisplatin and MT. The complex formation was consequently confirmed by quartz crystal microbalance analyses. The formation of this complex was detectable even after a 20 s long interaction. Moreover, we detected presence of MT-cisplatin complex in the blood of male rats treated with this drug.

Keywords

Cancer; Metallothionein; Cisplatin; Protein-Drug Interaction; Voltammetry; Brdicka's reaction; Quartz Crystal Microbalance

Authors

HÚSKA, D.; FABRIK, I.; BALOUN, J.; ADAM, V.; MASAŘÍK, M.; HUBÁLEK, J.; VAŠKŮ, A.; TRNKOVÁ, L.; HORNA, A.; ZEMAN, L.; KIZEK, R.

RIV year

2009

Released

15. 3. 2009

Publisher

MDPI

Location

MATTHAEUSSTRASSE 11, CH-4057 BASEL, SWITZERLAND

ISBN

1424-8220

Periodical

SENSORS

Year of study

2009 (9)

Number

3

State

Swiss Confederation

Pages from

1355

Pages to

1369

Pages count

15

URL

http://www.mdpi.com/1424-8220/9/3/1355

Full text in the Digital Library

http://hdl.handle.net/11012/70083

BibTex

@article{BUT48743,
  author="HÚSKA, D. and FABRIK, I. and BALOUN, J. and ADAM, V. and MASAŘÍK, M. and HUBÁLEK, J. and VAŠKŮ, A. and TRNKOVÁ, L. and HORNA, A. and ZEMAN, L. and KIZEK, R.",
  title="Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance",
  journal="SENSORS",
  year="2009",
  volume="2009 (9)",
  number="3",
  pages="1355--1369",
  doi="10.3390/s90301355",
  issn="1424-8220",
  url="http://www.mdpi.com/1424-8220/9/3/1355"
}