Publication detail

Recording of monophasic action potentials during ischemia - reperfusion by optical method

NOVÁKOVÁ, M. BARDOŇOVÁ, J. PROVAZNÍK, I.

Original Title

Recording of monophasic action potentials during ischemia - reperfusion by optical method

English Title

Recording of monophasic action potentials during ischemia - reperfusion by optical method

Type

journal article - other

Language

Czech

Original Abstract

The present paper deals with new possibility how to study ischemia and reperfusion in rabbit isolated heart perfused according to Langendorff. Electrogram was recorded by touch-less method and coronary flow measured throughout the experiment. Moreover, monophasic action potentials (MAPs) were obtained by optical probe using voltage-sensitive dye di-4-ANEPPS. Each experiment consisted from five successive periods: stabilization (control), staining with the dye, washout of the dye, flow ischemia, reperfusion. During ischemic period, typical changes of electrogram were observed and they were accompanied by corresponding changes of MAPs. In conclusion, MAPs recorded by optical method can be used as a tool for studying ischemia - reperfusion changes in isolated rabbit hearts. However, since the effects of the dye to tissue itself are under intensive investigation and not fully elucidated yet, it is necessary to be somewhat cautious about the results obtained in this way.

English abstract

The present paper deals with new possibility how to study ischemia and reperfusion in rabbit isolated heart perfused according to Langendorff. Electrogram was recorded by touch-less method and coronary flow measured throughout the experiment. Moreover, monophasic action potentials (MAPs) were obtained by optical probe using voltage-sensitive dye di-4-ANEPPS. Each experiment consisted from five successive periods: stabilization (control), staining with the dye, washout of the dye, flow ischemia, reperfusion. During ischemic period, typical changes of electrogram were observed and they were accompanied by corresponding changes of MAPs. In conclusion, MAPs recorded by optical method can be used as a tool for studying ischemia - reperfusion changes in isolated rabbit hearts. However, since the effects of the dye to tissue itself are under intensive investigation and not fully elucidated yet, it is necessary to be somewhat cautious about the results obtained in this way.

Keywords

Isolated rabbit heart, Ischemia, Reperfusion, Monophasic action potential, Electrogram

Key words in English

Isolated rabbit heart, Ischemia, Reperfusion, Monophasic action potential, Electrogram

Authors

NOVÁKOVÁ, M.; BARDOŇOVÁ, J.; PROVAZNÍK, I.

RIV year

2007

Released

23. 12. 2007

Location

Brno

ISBN

1211-3395

Periodical

Scripta medica

Year of study

2007

Number

6

State

Czech Republic

Pages from

1

Pages to

13

Pages count

13

BibTex

@article{BUT45329,
  author="Marie {Nováková} and Jana {Kolářová} and Valentine {Provazník}",
  title="Recording of monophasic action potentials during ischemia - reperfusion by optical method",
  journal="Scripta medica",
  year="2007",
  volume="2007",
  number="6",
  pages="1--13",
  issn="1211-3395"
}