Publication detail

Spectrometric and Voltammetric Analysis of Urease: Nickel Nanoelectrode as an Electrochemical Sensor

HUBÁLEK, J. HRADECKÝ, J. ADAM, V. KRYŠTOFOVÁ, O. HÚSKA, D. MASAŘÍK, M. TRNKOVÁ, L. HORNA, A. KLOSOVÁ, K. ADÁMEK, M. ZEHNÁLEK, J. KIZEK, R.

Original Title

Spectrometric and Voltammetric Analysis of Urease: Nickel Nanoelectrode as an Electrochemical Sensor

English Title

Spectrometric and Voltammetric Analysis of Urease: Nickel Nanoelectrode as an Electrochemical Sensor

Type

journal article

Language

en

Original Abstract

Urease is the enzyme catalyzing the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is substrate-specific, which means that the enzyme catalyzes the hydrolysis of urea only. This feature is a basic diagnostic criterion used in the determination of many bacteria species. Most of the methods utilized for detection of urease are based on analysis of its enzyme activity (the hydrolysis of urea). The aim of this work was to detect urease indirectly by spectrometric method and directly by voltammetric methods. As spectrometric method we used is called indophenol assay.

English abstract

Urease is the enzyme catalyzing the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is substrate-specific, which means that the enzyme catalyzes the hydrolysis of urea only. This feature is a basic diagnostic criterion used in the determination of many bacteria species. Most of the methods utilized for detection of urease are based on analysis of its enzyme activity (the hydrolysis of urea). The aim of this work was to detect urease indirectly by spectrometric method and directly by voltammetric methods. As spectrometric method we used is called indophenol assay.

Keywords

urease, electrochemical methods, nanotechnology, nanotube, nickel electrode, hanging mercury drop electrode, spectrometry

RIV year

2007

Released

16.07.2007

Publisher

MDPI

Pages from

1238

Pages to

1255

Pages count

18

URL

Full text in the Digital Library

BibTex


@article{BUT44511,
  author="Jaromír {Hubálek} and Kateřina {Klosová} and Martin {Adámek}",
  title="Spectrometric and Voltammetric Analysis of Urease: Nickel Nanoelectrode as an Electrochemical Sensor",
  annote="Urease is the enzyme catalyzing the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is substrate-specific, which means that the enzyme catalyzes the hydrolysis of urea only. This feature is a basic diagnostic criterion used in the determination of many bacteria species. Most of the methods utilized for detection of urease are based on analysis of its enzyme activity (the hydrolysis of urea). The aim of this work was to detect urease indirectly by spectrometric method and directly by voltammetric methods. As spectrometric method we used is called indophenol assay.",
  address="MDPI",
  chapter="44511",
  doi="10.3390/s7071238",
  institution="MDPI",
  journal="SENSORS",
  number="1",
  volume="7",
  year="2007",
  month="july",
  pages="1238--1255",
  publisher="MDPI",
  type="journal article"
}