Publication detail

Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy

ZIKMUND, T. KVASNICA, L. LOVICAR, L. UHLÍŘOVÁ, H. CHMELÍK, R.

Original Title

Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy

Type

conference paper

Language

English

Original Abstract

Digital holographic microscopy (DHM) is a powerful tool for observation of biological samples. The main advantage of DHM imaging is the possibility to reconstruct the intensity image and the phase image of an observed sample in real-time. The background of phase images is deformed and this deformation is changing during a long-term experiment. We describe here a novel procedure of deformation compensation for sequence of phase images. This novel procedure is based on deformation compensation procedure for a single phase image which uses the least square method. The novel procedure is applicable to compensate deformation during observation in real-time. The proposed procedure is applied to phase images of cells obtained by a transmitted-light digital holographic microscope.

Keywords

digital holographic microscopy, phase image, deformation compensation, least square method

Authors

ZIKMUND, T.; KVASNICA, L.; LOVICAR, L.; UHLÍŘOVÁ, H.; CHMELÍK, R.

RIV year

2010

Released

14. 12. 2010

Publisher

SPIE

ISBN

9780819482365

Book

Proceedings of SPIE, Volume 7746

ISBN

0277-786X

Periodical

Proceedings of SPIE

State

United States of America

Pages from

77460K-1

Pages to

77460K-7

Pages count

7