Publication detail

Electrophysiological effects of Voltage-Sensitive Dye di-4-ANEPPS in Rabbit and Guinea Pig Isolated Heart

FIALOVÁ, K. KOLÁŘOVÁ, J. SEKORA, J. TANNENBERG, M. NOVÁKOVÁ, M. PROVAZNÍK, I.

Original Title

Electrophysiological effects of Voltage-Sensitive Dye di-4-ANEPPS in Rabbit and Guinea Pig Isolated Heart

Type

conference paper

Language

English

Original Abstract

Voltage-sensitive dye (VSD) di-4-ANEPPS is currently used in our laboratory for recording of monophasic action potentials (MAPs) in isolated hearts. In this study, its electrophysiological effects on rabbit and guinea pig myocardium were compared during staining with VSD and washout from it. Electrograms were recorded and heart rate changes and the types and frequency of arrhythmias were evaluated. Althought certain electrophysiological changes were present in the hearts of both species, these changes are mostly reversible, especially in rabbit hearts. Thus, rabbit myocardium can be considered a reliable model for electrophysiological studies, especially when generation and propagation of the impulse is studied. It may be concluded that rabbit isolated heart is more suitable model for electrophysiological studies in the isolated heart model than guinea pig heart.

Keywords

Voltage-sensitive dye (VSD) di-4-ANEPPS, electrogram

Authors

FIALOVÁ, K.; KOLÁŘOVÁ, J.; SEKORA, J.; TANNENBERG, M.; NOVÁKOVÁ, M.; PROVAZNÍK, I.

RIV year

2010

Released

30. 6. 2010

Publisher

Brno University of Technology

Location

Brno

ISBN

978-80-214-4106-4

Book

Proceedings of Biosignal 2010: Analysis of Biomedical Signals and Images

Edition number

20

Pages from

108

Pages to

113

Pages count

6

BibTex

@inproceedings{BUT35735,
  author="Kateřina {Fialová} and Jana {Kolářová} and Jiří {Sekora} and Milan {Tannenberg} and Marie {Nováková} and Valentine {Provazník}",
  title="Electrophysiological effects of Voltage-Sensitive Dye di-4-ANEPPS in Rabbit and Guinea Pig Isolated Heart",
  booktitle="Proceedings of Biosignal 2010: Analysis of Biomedical Signals and Images",
  year="2010",
  number="20",
  pages="108--113",
  publisher="Brno University of Technology",
  address="Brno",
  isbn="978-80-214-4106-4"
}