Publication detail

Perimeter measurement of spruce needles profile using MRI

MIKULKA, J. GESCHEIDTOVÁ, E. BARTUŠEK, K.

Original Title

Perimeter measurement of spruce needles profile using MRI

English Title

Perimeter measurement of spruce needles profile using MRI

Type

conference paper

Language

en

Original Abstract

The paper describes using of MRI methods to assess the perimeter of the profile of spruce needles. The aim of this work is to create of multi-instrumental equipment for research on heavy metal (cadmium and lead) influence on development of spruce needles cellular germ (somatic embryos). During the research were observed differences in germ growth with different concentration of cadmium. With a certain concentration of cadmium the perimeter of needle increases more than with other. It is due to more intensive water usage from cultivation medium and then attenuation of heavy metal concentration inside the needle. As one of the observational methods of the mentioned phenomenon, was chosen analysis of the NMR image susceptible to estimation of the needle growth by the perimeter of the needle profile. Slices of the spruce needles were observed on MR tomograph UPT AV CR with the induction of magnetic field 4.7 T. Concurrently a method in AF MZLU was applied by cutting the needles on hand microtome, the images of the slices were observed by a digital camera with 1600x1200 pixels resolution and by using a binocular magnifying glass. The aim of the article is to compare of experimental results observed by both mentioned methods.

English abstract

The paper describes using of MRI methods to assess the perimeter of the profile of spruce needles. The aim of this work is to create of multi-instrumental equipment for research on heavy metal (cadmium and lead) influence on development of spruce needles cellular germ (somatic embryos). During the research were observed differences in germ growth with different concentration of cadmium. With a certain concentration of cadmium the perimeter of needle increases more than with other. It is due to more intensive water usage from cultivation medium and then attenuation of heavy metal concentration inside the needle. As one of the observational methods of the mentioned phenomenon, was chosen analysis of the NMR image susceptible to estimation of the needle growth by the perimeter of the needle profile. Slices of the spruce needles were observed on MR tomograph UPT AV CR with the induction of magnetic field 4.7 T. Concurrently a method in AF MZLU was applied by cutting the needles on hand microtome, the images of the slices were observed by a digital camera with 1600x1200 pixels resolution and by using a binocular magnifying glass. The aim of the article is to compare of experimental results observed by both mentioned methods.

Keywords

NMR, perimeter measurement, image processing

RIV year

2009

Released

23.03.2009

Publisher

The Electromagnetic Academy

Location

Cambridge

ISBN

978-1-934142-08-0

Book

Progress in Elekctromagnetic Research Symposium

Pages from

1075

Pages to

1078

Pages count

4

URL

Documents

BibTex


@inproceedings{BUT31858,
  author="Jan {Mikulka} and Eva {Gescheidtová} and Karel {Bartušek}",
  title="Perimeter measurement of spruce needles profile using MRI",
  annote="The paper describes using of MRI methods to assess the perimeter of the profile of spruce needles. The aim of this work is to create of multi-instrumental equipment for research on heavy metal (cadmium and lead) influence on development of spruce needles cellular germ (somatic embryos). During the research were observed differences in germ growth with different concentration of cadmium. With a certain concentration of cadmium the perimeter of needle increases more than with other. It is due to more intensive water usage from cultivation medium and then attenuation of heavy metal concentration inside the needle. As one of the observational methods of the mentioned phenomenon, was chosen analysis of the NMR image susceptible to estimation of the needle growth by the perimeter of the needle profile. Slices of the spruce needles were observed on MR tomograph UPT AV CR with the induction of magnetic field 4.7 T. Concurrently a method in AF MZLU was applied by cutting the needles on hand microtome, the images of the slices were observed by a digital camera with 1600x1200 pixels resolution and by using a binocular magnifying glass. The aim of the article is to compare of experimental results observed by both mentioned methods.",
  address="The Electromagnetic Academy",
  booktitle="Progress in Elekctromagnetic Research Symposium",
  chapter="31858",
  howpublished="electronic, physical medium",
  institution="The Electromagnetic Academy",
  year="2009",
  month="march",
  pages="1075--1078",
  publisher="The Electromagnetic Academy",
  type="conference paper"
}