Publication detail

MY_UMI_TOOL: Processing of Unique Molecular Identifiers without Mapping to a Reference Genome

BARILÍKOVÁ, L.

Original Title

MY_UMI_TOOL: Processing of Unique Molecular Identifiers without Mapping to a Reference Genome

Type

conference paper

Language

English

Original Abstract

The presented paper describes a novel algorithm for processing unique molecular identifiers (UMIs) without mapping to a reference genome. For the past few years, there has been a rapid rise in the use of sequencing technologies and progress in bioinformatics tools. However, available tools for processing UMIs are usually relative computationally demanding and time-consuming which led us to design and develop a new algorithm as well as to implement some available software to provide an effective estimation of the total number of unique molecules. My_UMI_tool performs four major steps: preprocessing of an input file, clustering by UMIs, clustering by sequence similarity, and marking duplicates to generate final file.

Keywords

unique molecular identifiers (UMIs); next-generation sequencing (NGS); PCR error

Authors

BARILÍKOVÁ, L.

Released

23. 4. 2020

Publisher

Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií

Location

Brno

ISBN

978-80-214-5868-0

Book

PROCEEDINGS II OF THE 26TH CONFERENCE STUDENT EEICT 2020

Edition number

1

Pages from

76

Pages to

79

Pages count

4

URL

https://www.fekt.vut.cz/conf/EEICT/archiv/sborniky/EEICT_2020_sbornik_2.pdf

BibTex

@inproceedings{BUT167748,
  author="Lujza {Barilíková}",
  title="MY_UMI_TOOL: Processing of Unique Molecular Identifiers without Mapping to a Reference Genome",
  booktitle="PROCEEDINGS II OF THE 26TH CONFERENCE STUDENT EEICT 2020",
  year="2020",
  number="1",
  pages="76--79",
  publisher="Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií",
  address="Brno",
  isbn="978-80-214-5868-0",
  url="https://www.fekt.vut.cz/conf/EEICT/archiv/sborniky/EEICT_2020_sbornik_2.pdf"
}