Publication detail

Deeper below the surface – transcriptional changes of selected Clostridium beijerinckii NRRL B-598 genes induced by butanol shock

PATÁKOVÁ, P. KOLEK, J. JUREČKOVÁ, K. BRANSKÁ, B. SEDLÁŘ, K. VASYLKIVSKA, M. PROVAZNÍK, I.

Original Title

Deeper below the surface – transcriptional changes of selected Clostridium beijerinckii NRRL B-598 genes induced by butanol shock

English Title

Deeper below the surface – transcriptional changes of selected Clostridium beijerinckii NRRL B-598 genes induced by butanol shock

Type

journal article in Web of Science

Language

en

Original Abstract

The main bottleneck in the return of industrial butanol production from renewable feedstock, by acetone-butanol-ethanol (ABE) fermentation by clostridia, such as Clostridium beijerinckii, is the low final butanol concentration. The problem is caused by high toxicity of butanol to the production cells, and therefore, understanding the mechanisms by which clostridia react to butanol shock is of key importance. Detailed analyses of transcriptome data obtained after butanol shock, and their comparison with data from standard ABE fermentations resulted in new findings while they confirmed expected population responses as well. Although butanol shock resulted in upregulation of heat shock protein genes, their regulation is probably different than was assumed based on standard ABE fermentation transcriptome data. While glucose uptake, glycolysis and acidogenesis genes were downregulated after butanol shock, solventogenesis genes were upregulated. Cyclopropanation of fatty acids and formation of plasmalogens seem to be significant processes involved in cell membrane stabilization in the presence of butanol. Surprisingly, one of three identified Agr quorum sensing system genes was upregulated. Upregulation of several putative butanol efflux pumps was described after butanol addition and a large putative polyketide gene cluster was found, the transcription of which seemed to be dependent on the concentration of butanol.

English abstract

The main bottleneck in the return of industrial butanol production from renewable feedstock, by acetone-butanol-ethanol (ABE) fermentation by clostridia, such as Clostridium beijerinckii, is the low final butanol concentration. The problem is caused by high toxicity of butanol to the production cells, and therefore, understanding the mechanisms by which clostridia react to butanol shock is of key importance. Detailed analyses of transcriptome data obtained after butanol shock, and their comparison with data from standard ABE fermentations resulted in new findings while they confirmed expected population responses as well. Although butanol shock resulted in upregulation of heat shock protein genes, their regulation is probably different than was assumed based on standard ABE fermentation transcriptome data. While glucose uptake, glycolysis and acidogenesis genes were downregulated after butanol shock, solventogenesis genes were upregulated. Cyclopropanation of fatty acids and formation of plasmalogens seem to be significant processes involved in cell membrane stabilization in the presence of butanol. Surprisingly, one of three identified Agr quorum sensing system genes was upregulated. Upregulation of several putative butanol efflux pumps was described after butanol addition and a large putative polyketide gene cluster was found, the transcription of which seemed to be dependent on the concentration of butanol.

Keywords

Clostridium beijerinckii; transcriptome analysis; ABE fermentation; butanol shock

Released

14.12.2020

Publisher

John Wiley & Sons

ISBN

2045-8827

Periodical

MicrobiologyOpen

Year of study

00

State

US

Pages from

1

Pages to

14

Pages count

14

URL

Full text in the Digital Library

Documents

BibTex


@article{BUT166224,
  author="Petra {Patáková} and Jan {Kolek} and Kateřina {Jurečková} and Barbora {Branská} and Karel {Sedlář} and Maryna {Vasylkivska} and Ivo {Provazník}",
  title="Deeper below the surface – transcriptional changes of selected Clostridium beijerinckii NRRL B-598 genes induced by butanol shock",
  annote="The main bottleneck in the return of industrial butanol production from renewable feedstock, by acetone-butanol-ethanol (ABE) fermentation by clostridia, such as Clostridium beijerinckii, is the low final butanol concentration. The problem is caused by high toxicity of butanol to the production cells, and therefore, understanding the mechanisms by which clostridia react to butanol shock is of key importance. Detailed analyses of transcriptome data obtained after butanol shock, and their comparison with data from standard ABE fermentations resulted in new findings while they confirmed expected population responses as well. Although butanol shock resulted in upregulation of heat shock protein genes, their regulation is probably different than was assumed based on standard ABE fermentation transcriptome data. While glucose uptake, glycolysis and acidogenesis genes were downregulated after butanol shock, solventogenesis genes were upregulated. Cyclopropanation of fatty acids and formation of plasmalogens seem to be significant processes involved in cell membrane stabilization in the presence of butanol. Surprisingly, one of three identified Agr quorum sensing system genes was upregulated. Upregulation of several putative butanol efflux pumps was described after butanol addition and a large putative polyketide gene cluster was found, the transcription of which seemed to be dependent on the concentration of butanol.",
  address="John Wiley & Sons",
  chapter="166224",
  doi="10.1002/mbo3.1146",
  howpublished="online",
  institution="John Wiley & Sons",
  number="0",
  volume="00",
  year="2020",
  month="december",
  pages="1--14",
  publisher="John Wiley & Sons",
  type="journal article in Web of Science"
}