Publication detail

The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond

Zhu, HL. Zhang, HQ. Ni, S. Korabecna, M. Yobas, L. Neuzil, P.

Original Title

The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond

English Title

The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond

Type

journal article in Web of Science

Language

en

Original Abstract

Infectious diseases, such as the most recent case of coronavirus disease 2019, have brought the prospect of point-of-care (POC) diagnostic tests into the spotlight. A rapid, accurate, low-cost, and easy-to-use test in the field could stop epidemics before they develop into full-blown pandemics. Unfortunately, despite all the advances, it still does not exist. Here, we critically review the limited number of prototypes demonstrated to date that is based on a polymerase chain reaction (PCR) and has come close to fulfill this vision. We summarize the requirements for the POC PCR tests and then go on to discuss the PCR product-detection methods, the integration of their functional components, the potential applications, and other practical issues related to the implementation of lab-on-a-chip technologies. We conclude our review with a discussion of the latest findings on nucleic acid-based diagnosis. (C) 2020 Elsevier B.V. All rights reserved.

English abstract

Infectious diseases, such as the most recent case of coronavirus disease 2019, have brought the prospect of point-of-care (POC) diagnostic tests into the spotlight. A rapid, accurate, low-cost, and easy-to-use test in the field could stop epidemics before they develop into full-blown pandemics. Unfortunately, despite all the advances, it still does not exist. Here, we critically review the limited number of prototypes demonstrated to date that is based on a polymerase chain reaction (PCR) and has come close to fulfill this vision. We summarize the requirements for the POC PCR tests and then go on to discuss the PCR product-detection methods, the integration of their functional components, the potential applications, and other practical issues related to the implementation of lab-on-a-chip technologies. We conclude our review with a discussion of the latest findings on nucleic acid-based diagnosis. (C) 2020 Elsevier B.V. All rights reserved.

Keywords

COVID-19 diagnoses; Polymerase chain reaction; Microfluidics; Miniaturization; Point of care; Future of PCR

Released

01.09.2020

Publisher

ELSEVIER SCI LTD

Location

OXFORD

ISBN

0165-9936

Periodical

TRAC-TRENDS IN ANALYTICAL CHEMISTRY

Year of study

130

Number

115984

State

GB

Pages from

115984

Pages to

115984

Pages count

13

URL

Documents

BibTex


@article{BUT165708,
  author="Pavel {Neužil}",
  title="The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond",
  annote="Infectious diseases, such as the most recent case of coronavirus disease 2019, have brought the prospect of point-of-care (POC) diagnostic tests into the spotlight. A rapid, accurate, low-cost, and easy-to-use test in the field could stop epidemics before they develop into full-blown pandemics. Unfortunately, despite all the advances, it still does not exist. Here, we critically review the limited number of prototypes demonstrated to date that is based on a polymerase chain reaction (PCR) and has come close to fulfill this vision. We summarize the requirements for the POC PCR tests and then go on to discuss the PCR product-detection methods, the integration of their functional components, the potential applications, and other practical issues related to the implementation of lab-on-a-chip technologies. We conclude our review with a discussion of the latest findings on nucleic acid-based diagnosis. (C) 2020 Elsevier B.V. All rights reserved.",
  address="ELSEVIER SCI LTD",
  chapter="165708",
  doi="10.1016/j.trac.2020.115984",
  howpublished="online",
  institution="ELSEVIER SCI LTD",
  number="115984",
  volume="130",
  year="2020",
  month="september",
  pages="115984--115984",
  publisher="ELSEVIER SCI LTD",
  type="journal article in Web of Science"
}