Publication detail

Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk

BEZDĚKOVÁ, J. VLČNOVSKÁ, M. ZEMÁNKOVÁ, K. BAČOVÁ, R. KOLÁČKOVÁ, M. LEDNICKÝ, T. PŘIBYL, J. RICHTERA, L. POMPEIANO VANÍČKOVÁ, L. ADAM, V. VACULOVIČOVÁ, M.

Original Title

Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk

Type

journal article in Web of Science

Language

English

Original Abstract

Dairy cow feed contains, among other ingredients, soybeans, legumes, and clover, plants that are rich in phytoestrogens. Several publications have reported a positive influence of phytoestrogens on human health; however, several unfavorable effects have also been reported. In this work, a simple, selective, and ecofriendly method of phytoestrogen isolation based on the technique of noncovalent molecular imprinting was developed. Genistein was used as a template, and dopamine was chosen as a functional monomer. A layer of molecularly imprinted polymers was created in a microtitration well plate. The binding capability and selective properties of obtained molecularly imprinted polymers were investigated. The imprinted polymers exhibited higher binding affinity toward chosen phytoestrogen than did the nonimprinted polymers. A selectivity factor of 6.94 was calculated, confirming satisfactory selectivity of the polymeric layer. The applicability of the proposed sensing method was tested by isolation of genistein from a real sample of bovine milk and combined with micellar electrokinetic capillary chromatography with UV-visible detection.

Keywords

biochanin A; genistein; dopamine; milk

Authors

BEZDĚKOVÁ, J.; VLČNOVSKÁ, M.; ZEMÁNKOVÁ, K.; BAČOVÁ, R.; KOLÁČKOVÁ, M.; LEDNICKÝ, T.; PŘIBYL, J.; RICHTERA, L.; POMPEIANO VANÍČKOVÁ, L.; ADAM, V.; VACULOVIČOVÁ, M.

Released

30. 6. 2020

ISBN

0022-0302

Periodical

JOURNAL OF DAIRY SCIENCE

Year of study

103

Number

6

State

United States of America

Pages from

4941

Pages to

4950

Pages count

10

URL

https://doi.org/10.3168/jds.2019-17367

BibTex

@article{BUT164384,
  author="Jaroslava {Bezděková} and Marcela {Vlčnovská} and Kristýna {Zemánková} and Romana {Bačová} and Martina {Koláčková} and Tomáš {Lednický} and Jan {Přibyl} and Lukáš {Richtera} and Lucie {Pompeiano Vaníčková} and Vojtěch {Adam} and Markéta {Vaculovičová}",
  title="Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk",
  journal="JOURNAL OF DAIRY SCIENCE",
  year="2020",
  volume="103",
  number="6",
  pages="4941--4950",
  doi="10.3168/jds.2019-17367",
  issn="0022-0302",
  url="https://doi.org/10.3168/jds.2019-17367"
}