Publication detail

Heat Transfer Time Determination Based on DNA Melting Curve Analysis

Hanliang Zhu, Huanan Li, Haoqing Zhang, Zdenka Fohlerova,Sheng Ni, Levent Yobas, Jaroslav Klempa, Imrich Gablech, Jaromir Hubalek, Honglong Chang, Pavel Neuzil

Original Title

Heat Transfer Time Determination Based on DNA Melting Curve Analysis

Type

journal article in Web of Science

Language

English

Original Abstract

The determination of the physical properties of fluids – such as the thermal characteristics, which include heat transfer time (Δt) – is becoming more challenging as system sizes shrink to micro and nanometer scales. Hence, knowledge of these properties is crucial for the operation of devices requiring precise temperature (T) control, such as polymerase chain reactions, melting curve analysis (MCA), and differential scanning fluorimetry. In this paper, we introduced a flow-through microfluidic system to analyze and compare thermal properties such as Δt among samples and the sidewall of a silicon chip using microscopic image analysis. We performed a spatial MCA with double-stranded deoxynucleic acid (dsDNA) and EvaGreen intercalator, using a flow-through microfluidic chip, and achieved a T gradient of ≈ 2.23 K·mm−1. We calculated the mean value of Δt as ≈ 33.9 ms from a melting temperature (TM) location shift along the microchannel for a variable flow rate. Our system had a T resolution of ≈ 1.2 mK·pixel-1 to distinguish different dsDNA molecules – based on the TM location within the chip – providing an option to use it as a high-throughput device for rapid DNA or protein analysis.

Keywords

dsDNA, melting curve analysis, heat transfer, microfluidics

Authors

Hanliang Zhu, Huanan Li, Haoqing Zhang, Zdenka Fohlerova,Sheng Ni, Levent Yobas, Jaroslav Klempa, Imrich Gablech, Jaromir Hubalek, Honglong Chang, Pavel Neuzil

Released

12. 1. 2020

ISBN

1613-4982

Periodical

Microfluidics and Nanofluidics

Number

10

State

Federal Republic of Germany

Pages from

1

Pages to

7

Pages count

7

URL

https://link.springer.com/article/10.1007/s10404-019-2308-9

BibTex

@article{BUT157899,
  author="Zdenka {Fohlerová} and Imrich {Gablech} and Jaromír {Hubálek} and Pavel {Neužil} and Jaroslav {Klempa}",
  title="Heat Transfer Time Determination Based on DNA Melting Curve Analysis",
  journal="Microfluidics and Nanofluidics",
  year="2020",
  number="10",
  pages="1--7",
  doi="10.1007/s10404-019-2308-9",
  issn="1613-4982",
  url="https://link.springer.com/article/10.1007/s10404-019-2308-9"
}