Publication detail

Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes

BUCHTOVÁ, Ž. LACKOVÁ, Z. KUDR, J. ZÍTKA, Z. ŠKODA, J. ZÍTKA, O.

Original Title

Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes

Type

journal article in Web of Science

Language

English

Original Abstract

Glutathione (gamma-glutamyl-cysteinyl-glycine; also known as GSH) is an endogenous antioxidant that plays a crucial role in cell defense mechanisms against oxidative stress. It is thus not surprising that this molecule can serve as a biomarker for oxidative stress monitoring. As capillary blood is a highly accessible target for biomarking, it is a valuable bodily fluid for diagnosing human GSH levels. This study focused on the optimization of GSH measurements from micro volumes of capillary blood prior to using electrochemical detection. The optimization of experimental parameters, including the sample volume and its stability, was performed and evaluated. Moreover, we tested the optimized method as part of a short-term study. The study consisted of examining 10 subjects within 96 h of their consumption of high amounts of antioxidants, attained from a daily dose of 2 g/150 mL of green tea. The subjects' capillary blood (5 mu L) was taken at 0 h, 48 h, and 96 h for subsequent analysis. The short-term supplementation of diet with green tea showed an increase of GSH pool by approximately 38% (between 0 and 48 h) within all subjects.

Keywords

antioxidant molecules; blood drop analysis; electrochemical analysis; nutritional study; sample pretreatment

Authors

BUCHTOVÁ, Ž.; LACKOVÁ, Z.; KUDR, J.; ZÍTKA, Z.; ŠKODA, J.; ZÍTKA, O.

Released

1. 10. 2018

Publisher

MDPI

ISBN

1420-3049

Periodical

MOLECULES

Year of study

23

Number

10

State

Swiss Confederation

Pages from

2504-1

Pages to

2504-10

Pages count

10

URL

Full text in the Digital Library