Publication detail

Effects of hydration of sodium 3-hydroxybutyrate on phase transitions connected to cryoprotectivity of microorganisms

SLANINOVÁ, E. SEDLÁČEK, P. OBRUČA, S. KOCHERBITOV, V.

Original Title

Effects of hydration of sodium 3-hydroxybutyrate on phase transitions connected to cryoprotectivity of microorganisms

Type

abstract

Language

English

Original Abstract

In this work, we are focused on cryoprotective mechanisms utilized by bacterial strain Cupriavidus necator H16 which produces and accumulates polyhydroxyalkanoates (PHA) in form of intracellular granules because of storage of carbon and energy and innate ability to protect itself against stress conditions. But intracellular granules do not obtain only PHA, due to metabolism of the PHA where PHA is simultaneously synthesized and degraded, the granules also contain its monomer 3-hydroxybutyrate (3HB).In our experimental part, we used differential scanning calorimetry (DSC) to observe the glass transitions and separated them into one independent and two dependent regions of phase diagram on water content. Also using the DSC data, we established the amount of nonfreezing water, which was calculated from linear regression (Figure 1) to be approx. 1,22g/g of monomer Na3HB. For determination of sorption isotherms of Na3HB with various content of water, we measured water activity by sorption calorimetry, dynamic vapor sorption and Novasina LabMaster which correspond to data obtained from DSC.

Keywords

sodium 3-hydroxybutyrate, cryoprotectant, water activity, phase diagram, DSC

Authors

SLANINOVÁ, E.; SEDLÁČEK, P.; OBRUČA, S.; KOCHERBITOV, V.

Released

12. 9. 2018

Publisher

Vysoké učení technické v Brně, Fakulta chemická

Location

Purkyňova 464/118, 612 00 Brno

ISBN

978-80-214-5488-0

Book

7th Meeting on Chemistry and Life 2018. Book of abstracts

Pages from

85

Pages to

85

Pages count

1

BibTex

@misc{BUT151418,
  author="SLANINOVÁ, E. and SEDLÁČEK, P. and OBRUČA, S. and KOCHERBITOV, V.",
  title="Effects of hydration of sodium 3-hydroxybutyrate on phase transitions connected to cryoprotectivity of microorganisms",
  booktitle="7th Meeting on Chemistry and Life 2018. Book of abstracts",
  year="2018",
  pages="85--85",
  publisher="Vysoké učení technické v Brně, Fakulta chemická",
  address="Purkyňova 464/118, 612 00 Brno",
  isbn="978-80-214-5488-0",
  note="abstract"
}