Publication detail

Novel Vancomycin-Peptide Conjugate as Potent Antibacterial Agent against Vancomycin-Resistant Staphylococcus aureus

JELÍNKOVÁ, P. ŠPLÍCHAL, Z. JIMENEZ JIMENEZ, A. HADDAD, Y. MAZUMDAR, A. SUR, V. MILOSAVLJEVIĆ, V. KOPEL, P. BUCHTELOVÁ, H. GURÁŇ, R. ZÍTKA, O. RICHTERA, L. HEGEROVÁ, D. HEGER, Z. MOULICK, A. ADAM, V.

Original Title

Novel Vancomycin-Peptide Conjugate as Potent Antibacterial Agent against Vancomycin-Resistant Staphylococcus aureus

Type

journal article in Web of Science

Language

English

Original Abstract

Background: Increase in vancomycin (Van)-resistant bacterial strains including vancomycin-resistant Staphylococcus aureus (VRSA) and lack of new effective antibiotics have become a formidable health problem. Materials and methods: We designed a new conjugate composed of Van and a peptide Hecate (Hec; Van/Hec), and its potential antimicrobial activity was evaluated. Results: Results from disk diffusion test, time-kill assay, determination of minimum inhibitory concentration (MIC), microscopy, and comet assay showed strong antimicrobial effects of Van/Hec against wild-type, methicillin-resistant Staphylococcus aureus (MRSA) and VRSA. Microscopy revealed that the exposure to Van/Hec results in disruption of bacterial cell integrity in all tested strains, which was not observed in case of Van or Hec alone. Conclusion: Overall, we showed that the preparation of conjugates from antibiotics and biologically active peptides could help us to overcome the limitation of the use of antibiotic in the treatment of infections caused by multidrug-resistant bacteria.

Keywords

Vancomycin; antibacterial; Staphylococcus aureus; antibiotic resistance; peptide

Authors

JELÍNKOVÁ, P.; ŠPLÍCHAL, Z.; JIMENEZ JIMENEZ, A.; HADDAD, Y.; MAZUMDAR, A.; SUR, V.; MILOSAVLJEVIĆ, V.; KOPEL, P.; BUCHTELOVÁ, H.; GURÁŇ, R.; ZÍTKA, O.; RICHTERA, L.; HEGEROVÁ, D.; HEGER, Z.; MOULICK, A.; ADAM, V.

Released

1. 11. 2018

Publisher

Dove Press

ISBN

1178-6973

Periodical

Infection and Drug Resistance

Year of study

11

Number

1

State

New Zealand

Pages from

1807

Pages to

1817

Pages count

11

URL

https://www.dovepress.com/novel-vancomycinndashpeptide-conjugate-as-potent-antibacterial-agent-a-peer-reviewed-article-IDR

Full text in the Digital Library

http://hdl.handle.net/11012/137077

BibTex

@article{BUT151171,
  author="Pavlína {Jelínková} and Zbyněk {Šplíchal} and Ana Maria {Jimenez Jimenez} and Yazan Abdulmajeed Eyadh {Haddad} and Aninda {Mazumdar} and Vishma Pratap {Sur} and Vedran {Milosavljević} and Pavel {Kopel} and Hana {Michálková} and Roman {Guráň} and Ondřej {Zítka} and Lukáš {Richtera} and Dagmar {Hegerová} and Zbyněk {Heger} and Amitava {Moulick} and Vojtěch {Adam}",
  title="Novel Vancomycin-Peptide Conjugate as Potent Antibacterial Agent against Vancomycin-Resistant Staphylococcus aureus",
  journal="Infection and Drug Resistance",
  year="2018",
  volume="11",
  number="1",
  pages="1807--1817",
  doi="10.2147/IDR.S160975",
  issn="1178-6973",
  url="https://www.dovepress.com/novel-vancomycinndashpeptide-conjugate-as-potent-antibacterial-agent-a-peer-reviewed-article-IDR"
}