Publication detail

Functional Analysis of Novicidin Peptide: Coordinated Delivery System for Zinc via Schiff Base Ligand

MILOSAVLJEVIĆ, V. HADDAD, Y. MOULICK, A. BUCHTELOVÁ, H. GURÁŇ, R. POSPÍŠIL, T. STOKOWA-SOLTYS, K. HEGER, Z. RICHTERA, L. KOPEL, P. ADAM, V.

Original Title

Functional Analysis of Novicidin Peptide: Coordinated Delivery System for Zinc via Schiff Base Ligand

Type

journal article in Web of Science

Language

English

Original Abstract

Novicidin (NVC), is a membrane-penetrating peptide, which forms a stable complex with Zn-Schiff base with interesting antitumor selectivity. We studied NVC derivatives to determine functional roles of key amino acids in toxicity, helicity, and binding of the Zn-Schiff base complex. Trimmed derivatives highlighted the role of peptide length and helicity in toxicity and membrane penetration. The removal of Lys from position 1 and 2 strongly increases the ability to disrupt the membranes. The trimming of the N-terminal residues significantly increases the stability of peptide helicity enhancing penetrating properties. Gly residue derivatives undermined a role of peptide bending in membrane penetration and toxicity. After the substitution of the central Gly derivatives with Ile or Lys, the peptides retained toxicity. These results illustrate the minor role of central helix bending in NVC toxicity. Binding-site-peptide derivatives identified His residue as the sole Zn-Schiff base binding site and eliminated the role of other aromatic residues.

Keywords

Novicidin (NVC); Zn-Schiff base complex

Authors

MILOSAVLJEVIĆ, V.; HADDAD, Y.; MOULICK, A.; BUCHTELOVÁ, H.; GURÁŇ, R.; POSPÍŠIL, T.; STOKOWA-SOLTYS, K.; HEGER, Z.; RICHTERA, L.; KOPEL, P.; ADAM, V.

Released

30. 9. 2018

ISBN

1043-1802

Periodical

BIOCONJUGATE CHEMISTRY

Year of study

29

Number

9

State

United States of America

Pages from

2954

Pages to

2969

Pages count

16

BibTex

@article{BUT150614,
  author="Vedran {Milosavljević} and Yazan Abdulmajeed Eyadh {Haddad} and Amitava {Moulick} and Hana {Michálková} and Roman {Guráň} and Tomáš {Pospíšil} and Kamila {Stokowa-Soltys} and Zbyněk {Heger} and Lukáš {Richtera} and Pavel {Kopel} and Vojtěch {Adam}",
  title="Functional Analysis of Novicidin Peptide: Coordinated Delivery System for Zinc via Schiff Base Ligand",
  journal="BIOCONJUGATE CHEMISTRY",
  year="2018",
  volume="29",
  number="9",
  pages="2954--2969",
  doi="10.1021/acs.bioconjchem.8b00370",
  issn="1043-1802"
}