Publication detail

Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion

TOLDE, O. GANDALOVIČOVÁ, A. KŘÍŽOVÁ, A. VESELÝ, P. CHMELÍK, R. RÖSEL, D. BRÁBEK, J.

Original Title

Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion

Type

journal article in Web of Science

Language

English

Original Abstract

Observation and analysis of cancer cell behaviour in 3D environment is essential for full understanding of the mechanisms of cancer cell invasion. However, label-free imaging of live cells in 3D conditions is optically more challenging than in 2D. Quantitative phase imaging provided by coherence controlled holographic microscopy produces images with enhanced information compared to ordinary light microscopy and, due to inherent coherence gate effect, enables observation of live cancer cells’ activity even in scattering milieu such as the 3D collagen matrix. Exploiting the dynamic phase differences method, we for the first time describe dynamics of differences in cell mass distribution in 3D migrating mesenchymal and amoeboid cancer cells, and also demonstrate that certain features are shared by both invasion modes. We found that amoeboid fibrosarcoma cells’ membrane blebbing is enhanced upon constriction and is also occasionally present in mesenchymally invading cells around constricted nuclei. Further, we demonstrate that both leading protrusions and leading pseudopods of invading fibrosarcoma cells are defined by higher cell mass density. In addition, we directly document bundling of collagen fibres by protrusions of mesenchymal fibrosarcoma cells. Thus, such a non-invasive microscopy offers a novel insight into cellular events during 3D invasion.

Keywords

Quantitative phase imaging , holographic microscopy, cancer cell invasion

Authors

TOLDE, O.; GANDALOVIČOVÁ, A.; KŘÍŽOVÁ, A.; VESELÝ, P.; CHMELÍK, R.; RÖSEL, D.; BRÁBEK, J.

Released

13. 8. 2018

Publisher

Springer Nature

ISBN

2045-2322

Periodical

Scientific Reports

Number

8

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

13

Pages count

13

URL

http://link.springer.com/article/10.1038/s41598-018-30408-7

Full text in the Digital Library

http://hdl.handle.net/11012/84157

BibTex

@article{BUT149217,
  author="Ondřej {Tolde} and Aneta {Gandalovičová} and Aneta {Křížová} and Pavel {Veselý} and Radim {Chmelík} and Daniel {Rösel} and Jan {Brábek}",
  title="Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion",
  journal="Scientific Reports",
  year="2018",
  number="8",
  pages="1--13",
  doi="10.1038/s41598-018-30408-7",
  issn="2045-2322",
  url="http://link.springer.com/article/10.1038/s41598-018-30408-7"
}