Publication detail

Determination of hydroxyproline using ion-exchange liquid chromatography with vis detector and high performance liquid chromatography with fluorescence detector

LACKOVÁ, Z. CERNEI, N. ŠTĚRBOVÁ, D. HADDAD, Y. ROZÍKOVÁ, V. KOMPRDA, T. ZÍTKA, O.

Original Title

Determination of hydroxyproline using ion-exchange liquid chromatography with vis detector and high performance liquid chromatography with fluorescence detector

Type

conference paper

Language

English

Original Abstract

The first aim of the experiment was an optimization of the method for determination of hydroxyproline in a pig skin using high-performance liquid chromatography with fluorescence detector (HPLC-FLD) and ion-exchange liquid chromatography with VIS detector (IEC-VIS). On the basis of the experiments performed, it was found that HPLC-FLD method is three times more sensitive than IEC-VIS method. For IEC analysis of hydroxyproline, the limit of detection (LOD) was 4.10 μg/ml whereas the limit of quantification (LOQ) was 13.50 μg/ml. For HPLC analysis of hydroxyproline, the limit of detection (LOD) was 1 ng/ml whereas the limit of quantification (LOQ) was 3 ng/ml. The second aim was the testing of influence of different volume of 6M HCl on extraction of sample (50 mg) for analysis using HPLC-FLD. Here it was found that the best volume was 250 µl 6M HCl.

Keywords

Hydroxyproline; HPLC-FLD; IEC-VIS; microwave hydrolysis; pig skin

Authors

LACKOVÁ, Z.; CERNEI, N.; ŠTĚRBOVÁ, D.; HADDAD, Y.; ROZÍKOVÁ, V.; KOMPRDA, T.; ZÍTKA, O.

Released

31. 12. 2017

Publisher

Mendel University in Brno

ISBN

978-80-7509-529-9

Book

MendelNet 2017

Pages from

905

Pages to

910

Pages count

6

URL

https://mendelnet.cz/artkey/mnt-201701-0104_Determination-of-hydroxyproline-using-ion-exchange-liquid-chromatography-with-VIS-detector-and-high-performance.php?back=/magno/mnt/2017/mn1.php?secid=4

Full text in the Digital Library

http://hdl.handle.net/11012/84181

BibTex

@inproceedings{BUT148747,
  author="Zuzana {Lacková} and Natalia Vladimirovna {Cernei} and Dagmar {Štěrbová} and Yazan Abdulmajeed Eyadh {Haddad} and Veronika {Rozíková} and Tomáš {Komprda} and Ondřej {Zítka}",
  title="Determination of hydroxyproline using ion-exchange liquid chromatography with vis detector and high performance liquid chromatography with fluorescence detector",
  booktitle="MendelNet 2017",
  year="2017",
  pages="905--910",
  publisher="Mendel University in Brno",
  isbn="978-80-7509-529-9",
  url="https://mendelnet.cz/artkey/mnt-201701-0104_Determination-of-hydroxyproline-using-ion-exchange-liquid-chromatography-with-VIS-detector-and-high-performance.php?back=/magno/mnt/2017/mn1.php?secid=4"
}