Publication detail

Electrochemical Study of DNA Damaged by Oxidation Stress

ZÍTKA, O. KŘÍŽKOVÁ, S. SKALIČKOVÁ, S. KOPEL, P. BABULA, P. ADAM, V. KIZEK, R.

Original Title

Electrochemical Study of DNA Damaged by Oxidation Stress

Type

journal article in Web of Science

Language

English

Original Abstract

Many compounds can interact with DNA leading to changes of DNA structure as point mutation and bases excision, which could trigger some metabolic failures, which leads to the changes in DNA structure resulting in cancer. Oxidation of nucleic acid bases belongs to the one of the mostly occurred type of DNA damaging leading to the above mentioned phenomena. The investigation of processes of DNA oxidation damage is topical and electrochemical methods include a versatile and sensitive tool for these purposes. 8-hydroxydeoxyguanosine (8-OHdG) is the most widely accepted marker of DNA damage. Oxidative damage to DNA by free radicals and exposure to ionizing radiation generate several other products within the double helix besides mentioned oxidation products of nucleic acid bases. The basic electrochemical behaviour of nucleic acids bases on various types of carbon electrodes is reviewed. Further, we address our attention on description of oxidation mechanisms and on detection of the most important products of nucleic bases oxidation. The miniaturization of detector coupled with some microfluidic devices is suggested and discussed.

Keywords

Miniaturization; nucleic acid bases; oxidation damage of DNA; voltammetry

Authors

ZÍTKA, O.; KŘÍŽKOVÁ, S.; SKALIČKOVÁ, S.; KOPEL, P.; BABULA, P.; ADAM, V.; KIZEK, R.

Released

1. 2. 2013

ISBN

1386-2073

Periodical

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

Year of study

16

Number

2

State

United Arab Emirates

Pages from

130

Pages to

141

Pages count

12

BibTex

@article{BUT143112,
  author="Ondřej {Zítka} and Soňa {Křížková} and Sylvie {Skaličková} and Pavel {Kopel} and Petr {Babula} and Vojtěch {Adam} and René {Kizek}",
  title="Electrochemical Study of DNA Damaged by Oxidation Stress",
  journal="COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING",
  year="2013",
  volume="16",
  number="2",
  pages="130--141",
  issn="1386-2073"
}