Publication detail

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

NEJDL, L. KUDR, J. MOULICK, A. HEGEROVÁ, D. RUTTKAY-NEDECKÝ, B. GUMULEC, J. ČÍHALOVÁ, K. ŠMERKOVÁ, K. DOSTÁLOVÁ, S. KŘÍŽKOVÁ, S. NOVOTNÁ, M. KOPEL, P. ADAM, V.

Original Title

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

Type

journal article in Web of Science

Language

English

Original Abstract

Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage λ, on the bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In vitro synthesized PtNPs were characterized by dynamic light scattering (PtNPs size range 4.8–11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry and atomic absorption spectrometry. The PtNPs inhibited the DNA replication and affected the secondary structure of DNA at higher concentrations, which was confirmed by polymerase chain reaction, DNA sequencing and DNA denaturation experiments. Further, cisplatin (CisPt), as traditional chemotherapy agent, was used in all parallel experiments. Moreover, the encapsulation of PtNPs in liposomes (LipoPtNPs) caused an approximately 2.4x higher of DNA damage in comparison with CisPt, LipoCisPt and PtNPs. The encapsulation of PtNPs in liposomes also increased their antibacterial, cytostatic and cytotoxic effect, which was determined by the method of growth curves on S. aureus and HFF cells. In addition, both the bare and encapsulated PtNPs caused lower oxidative stress (determined by GSH/GSSG ratio) in the human erythrocytes compared to the bare and encapsulated CisPt. CisPt was used in all parallel experiments as traditional chemotherapy agent.

Keywords

Platinum nanoparticles; DNA replication; DNA damage

Authors

NEJDL, L.; KUDR, J.; MOULICK, A.; HEGEROVÁ, D.; RUTTKAY-NEDECKÝ, B.; GUMULEC, J.; ČÍHALOVÁ, K.; ŠMERKOVÁ, K.; DOSTÁLOVÁ, S.; KŘÍŽKOVÁ, S.; NOVOTNÁ, M.; KOPEL, P.; ADAM, V.

Released

12. 7. 2017

Publisher

PLOS

ISBN

1932-6203

Periodical

PLOS ONE

Year of study

12

Number

7

State

United States of America

Pages from

1

Pages to

19

Pages count

19

URL

http://europepmc.org/articles/PMC5507526?pdf=render

Full text in the Digital Library

http://hdl.handle.net/11012/70092

BibTex

@article{BUT138521,
  author="Lukáš {Nejdl} and Jiří {Kudr} and Amitava {Moulick} and Dagmar {Hegerová} and Branislav {Ruttkay-Nedecký} and Jaromír {Gumulec} and Kristýna {Číhalová} and Kristýna {Šmerková} and Simona {Dostálová} and Soňa {Křížková} and Marie {Novotná} and Pavel {Kopel} and Vojtěch {Adam}",
  title="Platinum nanoparticles induce damage to DNA and inhibit DNA replication",
  journal="PLOS ONE",
  year="2017",
  volume="12",
  number="7",
  pages="1--19",
  doi="10.1371/journal.pone.0180798",
  issn="1932-6203",
  url="http://europepmc.org/articles/PMC5507526?pdf=render"
}