Publication detail

Automated classification of cell morphology by coherence-controlled holographic microscopy

ŠTRBKOVÁ, L. ZICHA, D. VESELÝ, P. CHMELÍK, R.

Original Title

Automated classification of cell morphology by coherence-controlled holographic microscopy

Type

journal article in Web of Science

Language

English

Original Abstract

In the last few years, classification of cells by machine learning has become frequently used in biology. However, most of the approaches are based on morphometric (MO) features, which are not quantitative in terms of cell mass. This may result in poor classification accuracy. Here, we study the potential contribution of coherence-controlled holographic microscopy enabling quantitative phase imaging for the classification of cell morphologies. We compare our approach with the commonly used method based on MO features. We tested both classification approaches in an experiment with nutritionally deprived cancer tissue cells, while employing several supervised machine learning algorithms. Most of the classifiers provided higher performance when quantitative phase features were employed. Based on the results, it can be concluded that the quantitative phase features played an important role in improving the performance of the classification. The methodology could be valuable help in refining the monitoring of live cells in an automated fashion. We believe that coherencecontrolled holographic microscopy, as a tool for quantitative phase imaging, offers all preconditions for the accurate automated analysis of live cell behavior while enabling noninvasive label-free imaging with sufficient contrast and high-spatiotemporal phase sensitivity.

Keywords

coherence-controlled holographic microscopy; digital holographic microscopy; quantitative phase imaging; supervised machine learning; classification; cell morphology

Authors

ŠTRBKOVÁ, L.; ZICHA, D.; VESELÝ, P.; CHMELÍK, R.

Released

23. 8. 2017

Publisher

SPIE

Location

Bellingham WA 98227-0010 USA

ISBN

1083-3668

Periodical

JOURNAL OF BIOMEDICAL OPTICS

Year of study

22

Number

8

State

United States of America

Pages from

086008-1

Pages to

086008-9

Pages count

9

URL

Full text in the Digital Library