Publication detail

Carbon Dots Based FRET for the Detection of DNA Damage

KUDR, J. RICHTERA, L. XHAXHIU, K. HYNEK, D. HEGER, Z. ZÍTKA, O. ADAM, V.

Original Title

Carbon Dots Based FRET for the Detection of DNA Damage

Type

journal article in Web of Science

Language

English

Original Abstract

Here, we aimed our attention at the synthesis of carbon dots (C-dots) with the ability to interact with DNA to suggest an approach for the detection of DNA damage. Primarily, C-dots modified with amine moieties were synthesized using the one-step microwave pyrolysis of citric acid in the presence of diethylenetriamine. The Cdots showed strong photoluminescence with a quantum yield of 4%. In addition, the C-dots (2.8 ± 0.8 nm) possessed a good colloidal stability and exhibited a positive surface charge (ζ=36 mV) at a neutral pH. An interaction study of the C-dots and the DNA fragment of λ bacteriophage was performed, and the DNA binding resulted in changes to the photoluminescent and absorption properties of the C-dots. A binding of the C-dots to DNA was also observed as a change to DNA electrophoretic mobility and a decreased ability to intercalate ethidium bromide (EtBr). Moreover, the Förster (or fluorescence) resonance energy transfer (FRET) between the C-dots and EtBr was studied, in which the C-dots serve as an excitation energy donor and the EtBr serves as an acceptor. When DNA was damaged using ultraviolet (UV) radiation (λ=254 nm) and hydroxyl radicals, the intensity of the emitted photoluminescence at 612 nm significantly decreased. The concept was proved on analysis of the genomic DNA from PC-3 cells and DNA isolated from melanoma tissues.

Keywords

Biosensing; Fluorescence resonance energy transfer; Quantum dots; UV irradiation

Authors

KUDR, J.; RICHTERA, L.; XHAXHIU, K.; HYNEK, D.; HEGER, Z.; ZÍTKA, O.; ADAM, V.

Released

15. 6. 2017

ISBN

0956-5663

Periodical

BIOSENSORS & BIOELECTRONICS

Year of study

92

Number

1

State

Kingdom of the Netherlands

Pages from

133

Pages to

139

Pages count

7

BibTex

@article{BUT136852,
  author="KUDR, J. and RICHTERA, L. and XHAXHIU, K. and HYNEK, D. and HEGER, Z. and ZÍTKA, O. and ADAM, V.",
  title="Carbon Dots Based FRET for the Detection of DNA Damage",
  journal="BIOSENSORS & BIOELECTRONICS",
  year="2017",
  volume="92",
  number="1",
  pages="133--139",
  doi="10.1016/j.bios.2017.01.067",
  issn="0956-5663"
}