Publication detail

Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA

MOULICK, A. MILOSAVLJEVIĆ, V. VLACHOVÁ, J. HYNEK, D. PODGAJNY, R. KOPEL, P. ADAM, V.

Original Title

Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA

Type

journal article in Web of Science

Language

English

Original Abstract

CdTe/ZnSe core/shell quantum dot (QD), one of the strongest and most highly luminescent nanoparticles, was directly synthesized in an aqueous medium to study its individual interactions with important nucleobases (adenine, guanine, cytosine, and thymine) in detail. The results obtained from the optical analyses indicated that the interactions of the QDs with different nucleobases were different, which reflected in different fluorescent emission maxima and intensities. The difference in the interaction was found due to the different chemical behavior and different sizes of the formed nanoconjugates. An electrochemical study also confirmed that the purines and pyrimidines show different interactions with the core/shell QDs. Based on these phenomena, a novel QD-based method is developed to detect the presence of the DNA, damage to DNA, and mutation. The QDs were successfully applied very easily to detect any change in the sequence (mutation) of DNA. The QDs also showed their ability to detect DNAs directly from the extracts of human cancer (PC3) and normal (PNT1A) cells (detection limit of 500 pM of DNA), which indicates the possibilities to use this easy assay technique to confirm the presence of living organisms in extreme environments.

Keywords

nanoparticles, nucleobases, biosensor, fluorescence, mutation

Authors

MOULICK, A.; MILOSAVLJEVIĆ, V.; VLACHOVÁ, J.; HYNEK, D.; PODGAJNY, R.; KOPEL, P.; ADAM, V.

Released

14. 2. 2017

Publisher

Dove Medical Press

ISBN

1178-2013

Periodical

International Journal of Nanomedicine

Year of study

12

Number

1

State

New Zealand

Pages from

1277

Pages to

1291

Pages count

15

URL

https://www.dovepress.com/using-cdteznse-coreshell-quantum-dots-to-detect-dna-and-damage-to-dna-peer-reviewed-fulltext-article-IJN

Full text in the Digital Library

http://hdl.handle.net/11012/193260

BibTex

@article{BUT134303,
  author="Amitava {Moulick} and Vedran {Milosavljević} and Jana {Vlachová} and David {Hynek} and Robert {Podgajny} and Pavel {Kopel} and Vojtěch {Adam}",
  title="Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA",
  journal="International Journal of Nanomedicine",
  year="2017",
  volume="12",
  number="1",
  pages="1277--1291",
  doi="10.2147/IJN.S121840",
  issn="1178-2013",
  url="https://www.dovepress.com/using-cdteznse-coreshell-quantum-dots-to-detect-dna-and-damage-to-dna-peer-reviewed-fulltext-article-IJN"
}