Publication detail

Superficial cells are self-renewing chondrocyte progenitors, which form the articular cartilage in juvenile mice

Li, L., Newton, P. T., Bouderlique, T., Sejnohova, M., Zikmund, T., Kozhemyakina, E., Xie, M., Krivanek, J., Kaiser, J., Qian, H., Dyachuk, V., Lassar, A. B., Warman, M. L., Barenius, B., Adameyko, I., Chagin, A. S.

Original Title

Superficial cells are self-renewing chondrocyte progenitors, which form the articular cartilage in juvenile mice

Type

journal article in Web of Science

Language

English

Original Abstract

Articular cartilage has little regenerative capacity. Recently, genetic lineage tracing experiments have revealed chondrocyte progenitors at the articular surface. We further characterized these progenitors by using in vivo genetic approaches. Histone H2B–green fluorescent protein retention revealed that superficial cells divide more slowly than underlying articular chondrocytes. Clonal genetic tracing combined with immunohistochemistry revealed that superficial cells renew their number by symmetric division, express mesenchymal stem cell markers, and generate chondrocytes via both asymmetric and symmetric differentiation. Quantitative analysis of cellular kinetics, in combination with phosphotungstic acid–enhanced micro–computed tomography,showed that superficial cells generate chondrocytes and contribute to the growth and reshaping of articular cartilage. Furthermore, we found that cartilage renewal occurs as the progeny of superficial cells fully replace fetal chondrocytes during early postnatal life. Thus, superficial cells are self-renewing progenitors that are capable of maintaining their own population and fulfilling criteria of unipotent adult stem cells. Furthermore, the progeny of these cells reconstitute adult articular cartilage de novo, entirely substituting fetal chondrocytes.

Keywords

superficial zone, bone, adult stem cells, regeneration, osteoarthritis

Authors

Li, L., Newton, P. T., Bouderlique, T., Sejnohova, M., Zikmund, T., Kozhemyakina, E., Xie, M., Krivanek, J., Kaiser, J., Qian, H., Dyachuk, V., Lassar, A. B., Warman, M. L., Barenius, B., Adameyko, I., Chagin, A. S.

Released

1. 3. 2017

ISBN

0892-6638

Periodical

The FASEB Journal

Year of study

31

Number

3

State

United States of America

Pages from

1067

Pages to

1084

Pages count

18

BibTex

@article{BUT130706,
  author="Jozef {Kaiser} and Tomáš {Zikmund} and Marie {Šejnohová}",
  title="Superficial cells are self-renewing chondrocyte progenitors, which form the articular cartilage in juvenile mice",
  journal="The FASEB Journal",
  year="2017",
  volume="31",
  number="3",
  pages="1067--1084",
  doi="10.1096/fj.201600918R",
  issn="0892-6638"
}