Publication detail

An evaluation of different coatings for TiN microelectrode chambers used for neonatal cardiomyocytes

SVOBODA, O. SKOPALÍK, J. BAIAZITOVA, L. GABRIELOVÁ, E. ČMIEL, V. PROVAZNÍK, I. FOHLEROVÁ, Z. HUBÁLEK, J.

Original Title

An evaluation of different coatings for TiN microelectrode chambers used for neonatal cardiomyocytes

English Title

An evaluation of different coatings for TiN microelectrode chambers used for neonatal cardiomyocytes

Type

conference paper

Language

en

Original Abstract

Neonatal myocytes are a widely used model for modern in-vitro pharmacological screening and tissue engineering of cardiac tissue. The recording of neonatal myocyte electrophysiology in real time has still not been ideally optimised, however. The aim of this study was to evaluate the impact of using different substrates in culture (MEA) chambers on the surviving and functional electrophysiological activity of the myocytes after the first five days after isolation. Neonatal cardiomyocytes were isolated from two day-old neonatal rats and plated on 12×12 ITO microelectrode chambers (Multi-Channel Systems) and cultured for five days. Four coating methods were used. The PDL + fibronectin treatment displayed the highest cardiomyocyte adhesion to the electrodes (20% of total cells in chamber after seeding) and developed contractible cells during the five days of pre-cultivation with a measurable electrical response. Nevertheless, the cells adhered to all surface-treated MEA chambers, creating typical cell morphology, but with different success rates.

English abstract

Neonatal myocytes are a widely used model for modern in-vitro pharmacological screening and tissue engineering of cardiac tissue. The recording of neonatal myocyte electrophysiology in real time has still not been ideally optimised, however. The aim of this study was to evaluate the impact of using different substrates in culture (MEA) chambers on the surviving and functional electrophysiological activity of the myocytes after the first five days after isolation. Neonatal cardiomyocytes were isolated from two day-old neonatal rats and plated on 12×12 ITO microelectrode chambers (Multi-Channel Systems) and cultured for five days. Four coating methods were used. The PDL + fibronectin treatment displayed the highest cardiomyocyte adhesion to the electrodes (20% of total cells in chamber after seeding) and developed contractible cells during the five days of pre-cultivation with a measurable electrical response. Nevertheless, the cells adhered to all surface-treated MEA chambers, creating typical cell morphology, but with different success rates.

Keywords

neonatal cardiomyocyte, microelectrode array, extracellular characterization

Released

14.09.2016

Publisher

Computing in Cardiology

Location

Vancouver, Canada

ISBN

978-1-5090-0684-7

Book

Computing in Cardiology 2016

Edition

43

Pages from

365

Pages to

368

Pages count

4

BibTex


@inproceedings{BUT128300,
  author="Ondřej {Svoboda} and Josef {Skopalík} and Larisa {Baiazitova} and Eva {Gabrielová} and Vratislav {Čmiel} and Ivo {Provazník} and Zdenka {Fohlerová} and Jaromír {Hubálek}",
  title="An evaluation of different coatings for TiN microelectrode chambers used for neonatal cardiomyocytes",
  annote="Neonatal myocytes are a widely used model for modern in-vitro pharmacological screening and tissue engineering of cardiac tissue. The recording of neonatal myocyte electrophysiology in real time has still not been ideally optimised, however. The aim of this study was to evaluate the impact of using different substrates in culture (MEA) chambers on the surviving and functional electrophysiological activity of the myocytes after the first five days after isolation. 
Neonatal cardiomyocytes were isolated from two day-old neonatal rats and plated on 12×12 ITO microelectrode chambers (Multi-Channel Systems) and cultured for five days.
Four coating methods were used. The PDL + fibronectin treatment displayed the highest cardiomyocyte adhesion to the electrodes (20% of total cells in chamber after seeding) and developed contractible cells during the five days of pre-cultivation with a measurable electrical response. Nevertheless, the cells adhered to all surface-treated MEA chambers, creating typical cell morphology, but with different success rates.",
  address="Computing in Cardiology",
  booktitle="Computing in Cardiology 2016",
  chapter="128300",
  edition="43",
  howpublished="online",
  institution="Computing in Cardiology",
  number="1",
  year="2016",
  month="september",
  pages="365--368",
  publisher="Computing in Cardiology",
  type="conference paper"
}