Publication detail

Chk1 inhibition significantly potentiates activity of nucleoside analogs in TP53-mutated B-lymphoid cells

ZEMANOVÁ, J. HYLSE, O. ČOLLÁKOVÁ, J. VESELÝ, P. OLTOVÁ, A. BORSKÝ, M. ZÁPRAŽNÁ, K. KAŠPÁRKOVÁ, M. JANOVSKÁ, P. VERNER, J. KOHOUTEK, J. DZIMKOVÁ, M. BRYJA, V. JAŠKOVÁ, Z. BRYCHTOVÁ, Y. PARUCH, K. TRBUŠEK, M.

Original Title

Chk1 inhibition significantly potentiates activity of nucleoside analogs in TP53-mutated B-lymphoid cells

Type

journal article in Web of Science

Language

English

Original Abstract

Treatment options for TP53-mutated lymphoid tumors are very limited. In experimental models, TP53-mutated lymphomas were sensitive to direct inhibition of checkpoint kinase 1 (Chk1), a pivotal regulator of replication. We initially tested the potential of the highly specific Chk1 inhibitor SCH900776 to synergize with nucleoside analogs (NAs) fludarabine, cytarabine and gemcitabine in cell lines derived from B-cell malignancies. In p53-proficient NALM-6 cells, SCH900776 added to NAs enhanced signaling towards Chk1 (pSer317/pSer345), effectively blocked Chk1 activation (Ser296 autophosphorylation), increased replication stress (p53 and γ-H2AX accumulation) and temporarily potentiated apoptosis. In p53-defective MEC-1 cell line representing adverse chronic lymphocytic leukemia (CLL), Chk1 inhibition together with NAs led to enhanced and sustained replication stress and significantly potentiated apoptosis. Altogether, among 17 tested cell lines SCH900776 sensitized four of them to all three NAs. Focusing further on MEC-1 and co-treatment of SCH900776 with fludarabine, we disclosed chromosome pulverization in cells undergoing aberrant mitoses. SCH900776 also increased the effect of fludarabine in a proportion of primary CLL samples treated with pro-proliferative stimuli, including those with TP53 disruption. Finally, we observed a fludarabine potentiation by SCH900776 in a T-cell leukemia 1 (TCL1)-driven mouse model of CLL. Collectively, we have substantiated the significant potential of Chk1 inhibition in B-lymphoid cells.

Keywords

checkpoint kinase 1/Chk1, SCH900776, nucleoside analogs, chronic lymphocytic leukemia, TP53

Authors

ZEMANOVÁ, J.; HYLSE, O.; ČOLLÁKOVÁ, J.; VESELÝ, P.; OLTOVÁ, A.; BORSKÝ, M.; ZÁPRAŽNÁ, K.; KAŠPÁRKOVÁ, M.; JANOVSKÁ, P.; VERNER, J.; KOHOUTEK, J.; DZIMKOVÁ, M.; BRYJA, V.; JAŠKOVÁ, Z.; BRYCHTOVÁ, Y.; PARUCH, K.; TRBUŠEK, M.

Released

19. 8. 2016

Publisher

Impact Journals

ISBN

1949-2553

Periodical

Oncotarget

Year of study

7

Number

38

State

United States of America

Pages from

1

Pages to

16

Pages count

16

URL

http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=11388

Full text in the Digital Library

http://hdl.handle.net/11012/84152

BibTex

@article{BUT127880,
  author="Jana {Zemanová} and Ondřej {Hylse} and Jana {Čolláková} and Pavel {Veselý} and Alexandra {Oltová} and Marek {Borský} and Kristína {Zápražná} and Marie {Kašpárková} and Pavlína {Janovská} and Jan {Verner} and Jiří {Kohoutek} and Marta {Dzimková} and Vítězslav {Bryja} and Zuzana {Jašková} and Yvona {Brychtová} and Kamil {Paruch} and Martin {Trbušek}",
  title="Chk1 inhibition significantly potentiates activity of nucleoside analogs in TP53-mutated B-lymphoid cells",
  journal="Oncotarget",
  year="2016",
  volume="7",
  number="38",
  pages="1--16",
  doi="10.18632/oncotarget.11388",
  issn="1949-2553",
  url="http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=11388"
}