Separation and detection of bioconjugated QDs in microfluidic devices using capillary electrophoresis

abstract

English

Semiconductor nanocrystals, also known as quantum dots (QDs), are nanoscale inorganic particles, and have been attract a lot of attention due to their unique optic and electronic properties. One of the most important characteristic is their bioconjugation with different biological materials such as protein, peptides and nucleic acid. Due to very diverse and numerous applications, it is really important to make a tool for precise and controlled detection of QDs. In this study, we investigate on-a-chip detection and separation of QDs. Separation is done by capillary electrophoresis using high voltage and as a carrier fluid, different buffers. Detection is done using UV filter and photodetector including high voltage photomultiplier. In first step mold, microchannels width of 50 µm was made using silicon wafer and photolithography. Next, mixture of the PDMS was completely degassed and pours onto the wafer mold to create transparent, flexible chip. Our first results showed that this method can be used to detected QDs, and separate QDs of different size as well as separate QDs bioconjugated with different biological material. Capillary electrophoresis is powerful separation technique that has been very useful in the analysis of biological material. What characterize this method are simplicity, short analysis time, small sample and reagent requirements, high separation efficient.

QDs, microfluidics, bioconjugation

PEJOVIC SIMEUNOVIC, J.; PEKÁRKOVÁ, J.; HUBÁLEK, J.

23. 4. 2015

Masarykova univerzita

Brno

978-80-210-7825-3

CEITEC PhD Retreat

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