Publication detail

Characterization of multi-walled carbon nanotubes double-functionalization with cytostatic drug etoposide and phosphorothioate oligodeoxynucleotides

HEGER, Z. MOULICK, A. NGUYEN, H. KREMPLOVÁ, M. KOPEL, P. HYNEK, D. ECKSCHLAGER, T. STIBOROVÁ, M. ZÍTKA, O. ADAM, V. KÍZEK, R.

Original Title

Characterization of multi-walled carbon nanotubes double-functionalization with cytostatic drug etoposide and phosphorothioate oligodeoxynucleotides

Type

journal article in Web of Science

Language

English

Original Abstract

Carbon nanomaterials possess unique structural and physicochemical properties, and thus they are broadly utilized as carriers for a variety of therapeutic agents in nanomedicinal applications. Herein we present an electrochemical characterization of binding capability of acidic oxidized multi-walled carbon nanotubes (oMWCNTs) modified with poly(ethylene glycol) towards common cytostatic drug etoposide (or VP-16). The comparison of willingness of oMWCNTs and MWCNTs-PEG to form complexes with etoposide revealed significantly higher capacity in PEGylated variant of carbon nanotubes (the 15 mM etoposide loading capacity was approximately 46.4% in 2 mg of MWCNT-PEG or 28.1% in equal amount of oMWCNT). To obtain a multifunctional nanotransporter we employed the phosphorothioate oligodeoxynucleotide (PODN), which could further extend the possible biological effects of MWCNT-PEG-Etoposide complex. By using square wave voltammetry, the binding capacity of 2 mg of MWCNT-PEG-Etoposide (15 mM) towards PODNs was determined to be 4.5 mu M. Such characterized multifunctional complex can be further employed for biological testing on chemoresistant tumors, such as non-small-cell lung cancer, to enhance the treatment efficiency.

Keywords

Antisense oligodeoxynucleotides; Carbon nanomaterials; Electrochemistry; Nanotechnology; Poly(ethylene) glycol

Authors

HEGER, Z.; MOULICK, A.; NGUYEN, H.; KREMPLOVÁ, M.; KOPEL, P.; HYNEK, D.; ECKSCHLAGER, T.; STIBOROVÁ, M.; ZÍTKA, O.; ADAM, V.; KÍZEK, R.

RIV year

2015

Released

1. 9. 2015

Publisher

ESG

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

10

Number

9

State

Republic of Serbia

Pages from

7707

Pages to

7719

Pages count

13

URL

http://www.electrochemsci.org/papers/vol10/100907707.pdf

Full text in the Digital Library

http://hdl.handle.net/11012/201457

BibTex

@article{BUT123363,
  author="Zbyněk {Heger} and Amitava {Moulick} and Hoai Viet {Nguyen} and Monika {Kremplová} and Pavel {Kopel} and David {Hynek} and Tomáš {Eckschlager} and Marie {Stiborová} and Ondřej {Zítka} and Vojtěch {Adam} and René {Kízek}",
  title="Characterization of multi-walled carbon nanotubes double-functionalization with cytostatic drug etoposide and phosphorothioate oligodeoxynucleotides",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2015",
  volume="10",
  number="9",
  pages="7707--7719",
  issn="1452-3981",
  url="http://www.electrochemsci.org/papers/vol10/100907707.pdf"
}