Publication detail

Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

KOLEK, J. SEDLÁŘ, K. PROVAZNÍK, I. PATÁKOVÁ, P.

Original Title

Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

Type

journal article in Web of Science

Language

English

Original Abstract

Butanol is currently one of the most discussed biofuels. Its use provides many benefits in comparison to bio-ethanol, but the price of its fermentative production is still high. Genetic improvements could help solve many problems associated with butanol production during ABE fermentation, such as its toxicity, low concentration achievable in the cultivation medium, the need for a relatively expensive substrate, and many more. Clostridium pasteurianum NRRL B-598 is non-type strain producing butanol, acetone, and a negligible amount of ethanol. Its main benefits are high oxygen tolerance, utilization of a wide range of carbon and nitrogen sources, and the availability of its whole genome sequence. However, there is no established method for the transfer of foreign DNA into this strain; this is the next step necessary for progress in its use for butanol production.

Keywords

Clostridium; Butanol; Transformation; Conjugation; Methylation; Dam; Dcm; Electroporation; Sonoporation

Authors

KOLEK, J.; SEDLÁŘ, K.; PROVAZNÍK, I.; PATÁKOVÁ, P.

Released

20. 1. 2016

Publisher

BioMed Central

ISBN

1754-6834

Periodical

BIOTECHNOL BIOFUELS

Year of study

9

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

14

Pages count

14

URL

http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0436-y

Full text in the Digital Library

http://hdl.handle.net/11012/69198

BibTex

@article{BUT120980,
  author="Jan {Kolek} and Karel {Sedlář} and Valentine {Provazník} and Petra {Patáková}",
  title="Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation",
  journal="BIOTECHNOL BIOFUELS",
  year="2016",
  volume="9",
  number="1",
  pages="1--14",
  doi="10.1186/s13068-016-0436-y",
  issn="1754-6834",
  url="http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0436-y"
}