Publication detail

The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy

ŠTRBKOVÁ, L. MANAKHOV, A. ZAJÍČKOVÁ, L. STOICA, A. VESELÝ, P. CHMELÍK, R.

Original Title

The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy

English Title

The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy

Type

journal article in Web of Science

Language

en

Original Abstract

The understanding of cell–surface interactions plays an important role for the biomaterials development and bioengineering. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of amine layers properties on cell viability is the subject of further investigation. In this work, amine-rich coatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency (RF) capacitively coupled discharge. Normal human dermal fibroblasts were chosen for the monitoring of biological response to the properties of amine layers. As a superior technique for the label-free monitoring of the cell–surface interaction, coherence-controlled holographic microscopy (CCHM) was exploited. CCHM enables quantitative phase imaging. From such images, valuable morphological parameters of cells directly related to the cell dry mass can be extracted. Based on those parameters, viability of cells cultivated on the plasmatreated surfaces with different properties was studied and evaluated. According to the results, amine-rich films enhanced the conditions for the cell adhesion and proliferation.

English abstract

The understanding of cell–surface interactions plays an important role for the biomaterials development and bioengineering. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of amine layers properties on cell viability is the subject of further investigation. In this work, amine-rich coatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency (RF) capacitively coupled discharge. Normal human dermal fibroblasts were chosen for the monitoring of biological response to the properties of amine layers. As a superior technique for the label-free monitoring of the cell–surface interaction, coherence-controlled holographic microscopy (CCHM) was exploited. CCHM enables quantitative phase imaging. From such images, valuable morphological parameters of cells directly related to the cell dry mass can be extracted. Based on those parameters, viability of cells cultivated on the plasmatreated surfaces with different properties was studied and evaluated. According to the results, amine-rich films enhanced the conditions for the cell adhesion and proliferation.

Keywords

Plasma polymer; cyclopropylamine; cell adhesion; holographic microscopy; quantitative phase imaging

Released

15.06.2016

Publisher

Elsevier B.V.

ISBN

0257-8972

Periodical

Surface and Coatings Technology

Year of study

295

Number

-

State

CH

Pages from

70

Pages to

77

Pages count

8

URL

Full text in the Digital Library

Documents

BibTex


@article{BUT120405,
  author="Lenka {Štrbková} and Anton {Manakhov} and Lenka {Zajíčková} and Adrian {Stoica} and Pavel {Veselý} and Radim {Chmelík}",
  title="The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy",
  annote="The understanding of cell–surface interactions plays an important role for the biomaterials development and bioengineering. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of amine layers properties on cell viability is the subject of further investigation. In this work, amine-rich coatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency (RF) capacitively coupled discharge. Normal human dermal fibroblasts were chosen for the monitoring of biological response to the properties of amine layers. As a superior technique for the label-free monitoring of the cell–surface interaction, coherence-controlled holographic microscopy (CCHM) was exploited. CCHM
enables quantitative phase imaging. From such images, valuable morphological parameters of cells directly related to the cell dry mass can be extracted. Based on those parameters, viability of cells cultivated on the plasmatreated surfaces with different properties was studied and evaluated. According to the results, amine-rich
films enhanced the conditions for the cell adhesion and proliferation.",
  address="Elsevier B.V.",
  chapter="120405",
  doi="10.1016/j.surfcoat.2015.10.076",
  howpublished="online",
  institution="Elsevier B.V.",
  number="-",
  volume="295",
  year="2016",
  month="june",
  pages="70--77",
  publisher="Elsevier B.V.",
  type="journal article in Web of Science"
}