Publication detail

Microfluidic Superheating for Peptide Sequence Elucidation

ALTMAYER, M. MANZ, A. NEUŽIL, P.

Original Title

Microfluidic Superheating for Peptide Sequence Elucidation

Type

journal article in Web of Science

Language

English

Original Abstract

Herein, we introduce microfluidic superheating as a new method for peptide fragmentation prior to mass spectrometric analysis. The superheating conditions were found to be stable up to 240 C for more than 30 min without elevated pressure or boiling of the aqueous sample. As proof of principle, we exposed the peptides ACTH1-10 and OVA257-264 to various superheating conditions, causing different degrees of decomposition. Optimized superheating conditions resulted in the entire peptide ladder sequence of the y-ions, allowing the amino acid sequence to be deduced from a single-stage mass spectrum. Thus, obtaining information in the same quality as from tandem mass spectrometry can be achieved by a single superheating step.

Keywords

superheating, peptides, hydrolysis

Authors

ALTMAYER, M.; MANZ, A.; NEUŽIL, P.

RIV year

2015

Released

2. 6. 2015

ISBN

0003-2700

Periodical

ANALYTICAL CHEMISTRY

Year of study

87

Number

12

State

United States of America

Pages from

5997

Pages to

6003

Pages count

7

BibTex

@article{BUT115137,
  author="Matthias O. {Altmayer} and Andreas {Manz} and Pavel {Neužil}",
  title="Microfluidic Superheating for Peptide Sequence Elucidation",
  journal="ANALYTICAL CHEMISTRY",
  year="2015",
  volume="87",
  number="12",
  pages="5997--6003",
  doi="10.1021/acs.analchem.5b00189",
  issn="0003-2700"
}