Publication detail

Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection

HEGER, Z. CERNEI, N. KŘÍŽKOVÁ, S. MASAŘÍK, M. KOPEL, P. HODEK, P. ZÍTKA, O. ADAM, V. KIZEK, R.

Original Title

Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection

Type

journal article in Web of Science

Language

English

Original Abstract

Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Forster resonance energy transfer (FRET). The FRET biosensor employs anti-sarcosine antibodies immobilized on paramagnetic nanoparticles surface for specific antigen binding. Successful binding of sarcosine leads to assembly of a sandwich construct composed of anti-sarcosine antibodies keeping the Forster distance (Ro) of FRET pair in required proximity. The detection is based on spectral overlap between gold-functionalized green fluorescent protein and antibodies@quantum dots bioconjugate (lex 400 nm). The saturation curve of sarcosine based on FRET efficiency (F604/F510 ratio) was tested within linear dynamic range from 5 to 50 nM with detection limit down to 50 pM. Assembled biosensor was then successfully employed for sarcosine quantification in prostatic cell lines (PC3, 22Rv1, PNT1A), and urinary samples of prostate adenocarcinoma patients.

Keywords

CANCER,DIAGNOSTIC MARKERS

Authors

HEGER, Z.; CERNEI, N.; KŘÍŽKOVÁ, S.; MASAŘÍK, M.; KOPEL, P.; HODEK, P.; ZÍTKA, O.; ADAM, V.; KIZEK, R.

RIV year

2015

Released

9. 3. 2015

Publisher

Nature Publishing Group

ISBN

2045-2322

Periodical

Scientific Reports

Year of study

5

Number

8868

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

7

Pages count

7

URL

http://www.nature.com/articles/srep08868

Full text in the Digital Library

http://hdl.handle.net/11012/63720

BibTex

@article{BUT113367,
  author="Zbyněk {Heger} and Natalia Vladimirovna {Cernei} and Soňa {Křížková} and Michal {Masařík} and Pavel {Kopel} and Petr {Hodek} and Ondřej {Zítka} and Vojtěch {Adam} and René {Kizek}",
  title="Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection",
  journal="Scientific Reports",
  year="2015",
  volume="5",
  number="8868",
  pages="1--7",
  doi="10.1038/srep08868",
  issn="2045-2322",
  url="http://www.nature.com/articles/srep08868"
}