Publication detail

Spectroscopic and Structural Analysis of Humic Substances Isolated from Forest Soil Humic Podzol - Locality Krkonoše, Czech Republic

ENEV, V. KLUČÁKOVÁ, M. NOVÁK, F.

Original Title

Spectroscopic and Structural Analysis of Humic Substances Isolated from Forest Soil Humic Podzol - Locality Krkonoše, Czech Republic

English Title

Spectroscopic and Structural Analysis of Humic Substances Isolated from Forest Soil Humic Podzol - Locality Krkonoše, Czech Republic

Type

conference paper

Language

en

Original Abstract

The aim of this work was study chemical composition, chemical properties and humification degree of different soil humic substances (HS). The object of our study were three various samples of HS. HAs and FA were isolated from forest soil Humic Podzol (locality Krkonoše, Czech Republic). Isolation of soil HS was performed according to the procedure recommended by the International Humic Substances Society (IHSS). All samples of soil HS were characterized by elemental analysis (EA), ultraviolet-visible spectroscopy (UV/Vis), Fourier transform infrared spectroscopy (FTIR) and steady-state fluorescence spectroscopy. Absorption coefficients (EET/EBz, E250/E365 and E465/E665) of soil HS were calculated from the absorbance values. Infrared spectroscopy is a useful technique in characterization of structure, functional groups and formation modes of HS. For the fluorescence experiments the final concentration of the HS was adjusted to 10 mg/dm3. The pH-value of the samples was adjusted to seven using a standard phosphate buffer. Fluorescence mono-dimensional spectra and total luminescence spectra (TLS) of soil HS were obtained using steady-state fluorescence spectroscopy. All fluorescence spectra were performed on a Horiba Scientific Fluorolog. Fluorescence index (Milori index and HIX) of HS was calculated from the area of the emission spectra. The fluorescence intensity (IF) values (in CPS) of samples were corrected using method of Lakowicz. Soil A55 HA was characterized high molecular weight, low molecular heterogeneity, high degree of aromatic polycondensation, high level of conjugated fluorophores, and high humification degree. HS isolated from organic horizon were characterized lower molecular mass, simple structural components of wide molecular heterogeneity, lower degree of aromatic polycondensation, and lower humification degree.

English abstract

The aim of this work was study chemical composition, chemical properties and humification degree of different soil humic substances (HS). The object of our study were three various samples of HS. HAs and FA were isolated from forest soil Humic Podzol (locality Krkonoše, Czech Republic). Isolation of soil HS was performed according to the procedure recommended by the International Humic Substances Society (IHSS). All samples of soil HS were characterized by elemental analysis (EA), ultraviolet-visible spectroscopy (UV/Vis), Fourier transform infrared spectroscopy (FTIR) and steady-state fluorescence spectroscopy. Absorption coefficients (EET/EBz, E250/E365 and E465/E665) of soil HS were calculated from the absorbance values. Infrared spectroscopy is a useful technique in characterization of structure, functional groups and formation modes of HS. For the fluorescence experiments the final concentration of the HS was adjusted to 10 mg/dm3. The pH-value of the samples was adjusted to seven using a standard phosphate buffer. Fluorescence mono-dimensional spectra and total luminescence spectra (TLS) of soil HS were obtained using steady-state fluorescence spectroscopy. All fluorescence spectra were performed on a Horiba Scientific Fluorolog. Fluorescence index (Milori index and HIX) of HS was calculated from the area of the emission spectra. The fluorescence intensity (IF) values (in CPS) of samples were corrected using method of Lakowicz. Soil A55 HA was characterized high molecular weight, low molecular heterogeneity, high degree of aromatic polycondensation, high level of conjugated fluorophores, and high humification degree. HS isolated from organic horizon were characterized lower molecular mass, simple structural components of wide molecular heterogeneity, lower degree of aromatic polycondensation, and lower humification degree.

Keywords

soil humic and fulvic acids, fluorescence, absorption and fluorescence indexes, chemical composition and structure, humification degree, O-containing functional groups

RIV year

2014

Released

04.12.2014

Publisher

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

Location

Brno

ISBN

978-80-214-5077-6

Book

Studentská odborná konference: Chemie je život 2014, Sborník příspěvků

Edition number

první

Pages from

216

Pages to

222

Pages count

7

Documents

BibTex


@inproceedings{BUT111324,
  author="Vojtěch {Enev} and Martina {Klučáková} and František {Novák}",
  title="Spectroscopic and Structural Analysis of Humic Substances Isolated from Forest Soil Humic Podzol - Locality Krkonoše, Czech Republic",
  annote="The aim of this work was study chemical composition, chemical properties and humification degree of different soil humic substances (HS). The object of our study were three various samples of HS. HAs and FA were isolated from forest soil Humic Podzol (locality Krkonoše, Czech Republic). Isolation of soil HS was performed according to the procedure recommended by the International Humic Substances Society (IHSS). All samples of soil HS were characterized by elemental analysis (EA), ultraviolet-visible spectroscopy (UV/Vis), Fourier transform infrared spectroscopy (FTIR) and steady-state fluorescence spectroscopy. Absorption coefficients (EET/EBz, E250/E365 and E465/E665) of soil HS were calculated from the absorbance values. Infrared spectroscopy is a useful technique in characterization of structure, functional groups and formation modes of HS. For the fluorescence experiments the final concentration of the HS was adjusted to 10 mg/dm3. The pH-value of the samples was adjusted to seven using a standard phosphate buffer. Fluorescence mono-dimensional spectra and total luminescence spectra (TLS) of soil HS were obtained using steady-state fluorescence spectroscopy. All fluorescence spectra were performed on a Horiba Scientific Fluorolog. Fluorescence index (Milori index and HIX) of HS was calculated from the area of the emission spectra. The fluorescence intensity (IF) values (in CPS) of samples were corrected using method of Lakowicz. Soil A55 HA was characterized high molecular weight, low molecular heterogeneity, high degree of aromatic polycondensation, high level of conjugated fluorophores, and high humification degree. HS isolated from organic horizon were characterized lower molecular mass, simple structural components of wide molecular heterogeneity, lower degree of aromatic polycondensation, and lower humification degree.",
  address="Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno",
  booktitle="Studentská odborná konference: Chemie je život 2014, Sborník příspěvků",
  chapter="111324",
  howpublished="electronic, physical medium",
  institution="Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno",
  year="2014",
  month="december",
  pages="216--222",
  publisher="Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno",
  type="conference paper"
}