Publication detail

Succession and Replacement of Bacterial Populations in the Caecum of Egg Laying Hens over Their Whole Life

VÍDEŇSKÁ, P. SEDLÁŘ, K. LUKAC, M. FALDYNOVÁ, M. GERŽOVÁ, L. ČEJKOVÁ, D. ŠIŠÁK, F. RYCHLÍK, I.

Original Title

Succession and Replacement of Bacterial Populations in the Caecum of Egg Laying Hens over Their Whole Life

Czech Title

Vývoj mikroflóry ve střevech nosnic během celého života

English Title

Succession and Replacement of Bacterial Populations in the Caecum of Egg Laying Hens over Their Whole Life

Type

journal article

Language

en

Original Abstract

In this study we characterised the development of caecal microbiota in egg laying hens over their commercial production lifespan, from the day of hatching until 60 weeks of age. Using pyrosequencing of V3/V4 variable regions of 16S rRNA genes for microbiota characterisation, we were able to define 4 different stages of caecal microbiota development. The first stage lasted for the first week of life and was characterised by a high prevalence of Enterobacteriaceae (phylum Proteobacteria). The second stage lasted from week 2 to week 4 and was characterised by nearly an absolute dominance of Lachnospiraceae and Ruminococcaceae (both phylum Firmicutes). The third stage lasted from month 2 to month 6 and was characterised by the succession of Firmicutes at the expense of Bacteroidetes. The fourth stage was typical for adult hens in full egg production aged 7 months or more and was characterised by a constant ratio of Bacteroidetes and Firmicutes formed by equal numbers of the representatives of both phyla.

Czech abstract

V této studii jsme charakterizovali vývoj mikroflóry ve střevech nosnic ode dne vylíhnutí až do uplynutí 60 týdnů věku. SekvenovánímV3 / V4 variabilních oblastí 16S rRNA genů pro charakterizaci mikroflóry, jsme byli schopni definovat 4 různé stupně vývoje mikroflóry. První fáze trvala po první týden života a byla charakterizována vysokou prevalencí Enterobacteriaceae (kmen Proteobacteria). Druhá etapa trvala od 2. týdne do 4. týdnu byla charakterizována téměř absolutní dominancí Lachnospiraceae a Ruminococcaceae (oba kmen Firmicutes). Třetí etapa trvala od druhého do šestého měsíce a byla charakterizována řadou Firmicutes na úkor Bacteroidetes. Čtvrtá etapa byla typická pro dospělé slepice a byla charakterizována konstantním poměrem Bacteroidetes a Firmicutes tvořenou stejného počtu zástupců obou kmenů.

English abstract

In this study we characterised the development of caecal microbiota in egg laying hens over their commercial production lifespan, from the day of hatching until 60 weeks of age. Using pyrosequencing of V3/V4 variable regions of 16S rRNA genes for microbiota characterisation, we were able to define 4 different stages of caecal microbiota development. The first stage lasted for the first week of life and was characterised by a high prevalence of Enterobacteriaceae (phylum Proteobacteria). The second stage lasted from week 2 to week 4 and was characterised by nearly an absolute dominance of Lachnospiraceae and Ruminococcaceae (both phylum Firmicutes). The third stage lasted from month 2 to month 6 and was characterised by the succession of Firmicutes at the expense of Bacteroidetes. The fourth stage was typical for adult hens in full egg production aged 7 months or more and was characterised by a constant ratio of Bacteroidetes and Firmicutes formed by equal numbers of the representatives of both phyla.

Keywords

metagenomics, chicken microbiota, 16S rRNA

RIV year

2014

Released

12.12.2014

Publisher

Public Library of Science

Location

California (US)

Pages from

1

Pages to

14

Pages count

14

URL

Full text in the Digital Library

BibTex


@article{BUT111135,
  author="Petra {Vídeňská} and Karel {Sedlář} and Maja {Lukac} and Marcela {Faldynová} and Lenka {Geržová} and Darina {Čejková} and František {Šišák} and Ivan {Rychlík}",
  title="Succession and Replacement of Bacterial Populations in the Caecum of Egg Laying Hens over Their Whole Life",
  annote="In this study we characterised the development of caecal microbiota in egg laying hens over their commercial production lifespan, from the day of hatching until 60 weeks of age. Using pyrosequencing of V3/V4 variable regions of 16S rRNA genes for microbiota characterisation, we were able to define 4 different stages of caecal microbiota development. The first stage lasted for the first week of life and was characterised by a high prevalence of Enterobacteriaceae (phylum Proteobacteria). The second stage lasted from week 2 to week 4 and was characterised by nearly an absolute dominance of Lachnospiraceae and Ruminococcaceae (both phylum Firmicutes). The third stage lasted from month 2 to month 6 and was characterised by the succession of Firmicutes at the expense of Bacteroidetes. The fourth stage was typical for adult hens in full egg production aged 7 months or more and was characterised by a constant ratio of Bacteroidetes and Firmicutes formed by equal numbers of the representatives of both phyla.",
  address="Public Library of Science",
  chapter="111135",
  doi="10.1371/journal.pone.0115142",
  howpublished="online",
  institution="Public Library of Science",
  number="12",
  volume="9",
  year="2014",
  month="december",
  pages="1--14",
  publisher="Public Library of Science",
  type="journal article"
}