Publication detail

Light Scattering Techniques Applied for the Study of Aging of Biopolymers and Biocolloids

KALINA, M. SMILEK, J. KLUČÁKOVÁ, M.

Original Title

Light Scattering Techniques Applied for the Study of Aging of Biopolymers and Biocolloids

English Title

Light Scattering Techniques Applied for the Study of Aging of Biopolymers and Biocolloids

Type

abstract

Language

en

Original Abstract

Biopolymers and biocolloids as natural product are often subjected to aggregation and degradation. Both these processes are extremely important and must be taken into account in the case of industrial application of these natural substances. Aggregated or degraded material used as a raw material for industrial production can significantly change the final yield and properties of product. This contribution is discussing the application of light scattering techniques for observation of stability of these compounds in time and for study of influence of different parameters (UV light, temperature, pH, ionic strength) on the stability. In experimental work proteins (bovine serum albumin, cystein) and polysaccharides (chitosan, glucose, sucrose, carboxymethylcellulose and sodium alginate) were used as model biopolymers. Biocolloids were represented by humic acids. Stability and degradation of proteins and polysaccharides was studied using zeta potential (method of electrophoretic light scattering) and molecular weight determination (combination of size exclusion chromatography with multiangle light scattering detection). Aging of humic acids was investigated by means of zeta potential differences in time and time development of particle size (method of dynamic light scattering). Results showed that light scattering techniques can be used as easy and quick methods for purposes of detection of the aggregation or degradation of biomaterials. These methods can be useful either in the case of checking of stored stock solutions, for continual controlling of raw materials for industrial production and also for detection of any deviations of final products.

English abstract

Biopolymers and biocolloids as natural product are often subjected to aggregation and degradation. Both these processes are extremely important and must be taken into account in the case of industrial application of these natural substances. Aggregated or degraded material used as a raw material for industrial production can significantly change the final yield and properties of product. This contribution is discussing the application of light scattering techniques for observation of stability of these compounds in time and for study of influence of different parameters (UV light, temperature, pH, ionic strength) on the stability. In experimental work proteins (bovine serum albumin, cystein) and polysaccharides (chitosan, glucose, sucrose, carboxymethylcellulose and sodium alginate) were used as model biopolymers. Biocolloids were represented by humic acids. Stability and degradation of proteins and polysaccharides was studied using zeta potential (method of electrophoretic light scattering) and molecular weight determination (combination of size exclusion chromatography with multiangle light scattering detection). Aging of humic acids was investigated by means of zeta potential differences in time and time development of particle size (method of dynamic light scattering). Results showed that light scattering techniques can be used as easy and quick methods for purposes of detection of the aggregation or degradation of biomaterials. These methods can be useful either in the case of checking of stored stock solutions, for continual controlling of raw materials for industrial production and also for detection of any deviations of final products.

Keywords

light scattering, biopolymers, biocolloids, particle size, zeta potential, molecular weight, aggregation, degradation

Released

28.10.2014

Publisher

Masaryk University

Location

Brno

ISBN

978-80-210-7159-9

Book

CEITEC Annual Conference - "Frontiers in Materials and Life Sciences"

Pages from

143

Pages to

143

Pages count

1

Documents

BibTex


@misc{BUT110153,
  author="Michal {Kalina} and Jiří {Smilek} and Martina {Klučáková}",
  title="Light Scattering Techniques Applied for the Study of Aging of Biopolymers and Biocolloids",
  annote="Biopolymers and biocolloids as natural product are often subjected to aggregation and degradation. Both these processes are extremely important and must be taken into account in the case of industrial application of these natural substances. Aggregated or degraded material used as a raw material for industrial production can significantly change the final yield and properties of product. This contribution is discussing the application of light scattering techniques for observation of stability of these compounds in time and for study of influence of different parameters (UV light, temperature, pH, ionic strength) on the stability. In experimental work proteins (bovine serum albumin, cystein) and polysaccharides (chitosan, glucose, sucrose, carboxymethylcellulose and sodium alginate) were used as model biopolymers. Biocolloids were represented by humic acids. Stability and degradation of proteins and polysaccharides was studied using zeta potential (method of electrophoretic light scattering) and molecular weight determination (combination of size exclusion chromatography with multiangle light scattering detection). Aging of humic acids was investigated by means of zeta potential differences in time and time development of particle size (method of dynamic light scattering). Results showed that light scattering techniques can be used as easy and quick methods for purposes of detection of the aggregation or degradation of biomaterials. These methods can be useful either in the case of checking of stored stock solutions, for continual controlling of raw materials for industrial production and also for detection of any deviations of final products.",
  address="Masaryk University",
  booktitle="CEITEC Annual Conference - "Frontiers in Materials and Life Sciences"",
  chapter="110153",
  institution="Masaryk University",
  year="2014",
  month="october",
  pages="143--143",
  publisher="Masaryk University",
  type="abstract"
}