Publication detail

Evaluation of Antioxidant Activity, Polyphenolic Compounds, Amino Acids and Mineral Elements of Representative Genotypes of Lonicera edulis

SOCHOR, J. JUŘÍKOVÁ, T. POHANKA, M. ŠKUTKOVÁ, H. BAROŇ, M. TOMÁŠKOVÁ, L. BALLA, Š. KLEJDUS, B. POKLUDA, R. MLČEK, J. TROJÁKOVÁ, Z. ŠALOUN, J.

Original Title

Evaluation of Antioxidant Activity, Polyphenolic Compounds, Amino Acids and Mineral Elements of Representative Genotypes of Lonicera edulis

English Title

Evaluation of Antioxidant Activity, Polyphenolic Compounds, Amino Acids and Mineral Elements of Representative Genotypes of Lonicera edulis

Type

journal article in Web of Science

Language

en

Original Abstract

The aim of this study was to evaluate the bioactive substances in 19 berry cultivars of edible honeysuckle (Lonicera edulis). A statistical evaluation was used to determine the relationship between the content of selected bioactive substances and individual cultivars. Regarding mineral elements, the content of sodium was measured using potentiometry and spectrophotometry. The content of selected polyphenolic compounds with high antioxidant activity was determined by a HPLC-UV/ED method. The total amount of polyphenols was determined by the Folin-Ciocalteu method. The antioxidant activity was determined using five methods (DPPH, FRAP, ABTS, FR and DMPD) that differ in their principles. The content of 13 amino acids was determined by ion-exchange chromatography. The experimental results obtained for the different cultivars were evaluated and compared by statistical and bioinformatic methods. A unique feature of this study lies in the exhaustive analysis of the chosen parameters (amino acids, mineral elements, polyphenolic compounds and antioxidant activity) during one growing season.

English abstract

The aim of this study was to evaluate the bioactive substances in 19 berry cultivars of edible honeysuckle (Lonicera edulis). A statistical evaluation was used to determine the relationship between the content of selected bioactive substances and individual cultivars. Regarding mineral elements, the content of sodium was measured using potentiometry and spectrophotometry. The content of selected polyphenolic compounds with high antioxidant activity was determined by a HPLC-UV/ED method. The total amount of polyphenols was determined by the Folin-Ciocalteu method. The antioxidant activity was determined using five methods (DPPH, FRAP, ABTS, FR and DMPD) that differ in their principles. The content of 13 amino acids was determined by ion-exchange chromatography. The experimental results obtained for the different cultivars were evaluated and compared by statistical and bioinformatic methods. A unique feature of this study lies in the exhaustive analysis of the chosen parameters (amino acids, mineral elements, polyphenolic compounds and antioxidant activity) during one growing season.

Keywords

Lonicera edulis; mineral compounds; phenolic compounds; antioxidant activity; amino acids; bioinformatics analysis

RIV year

2014

Released

21.05.2014

Publisher

MDPI

Location

SWITZERLAND

Pages from

6505

Pages to

6523

Pages count

20

URL

Full text in the Digital Library

BibTex


@article{BUT110091,
  author="Jiří {Sochor} and Tunde {Juříková} and Miroslav {Pohanka} and Helena {Škutková} and Mojmír {Baroň} and Lenka {Tomášková} and Štefan {Balla} and Bořivoj {Klejdus} and Robert {Pokluda} and Jiří {Mlček} and Zuzana {Trojáková} and Jan {Šaloun}",
  title="Evaluation of Antioxidant Activity, Polyphenolic Compounds, Amino Acids and Mineral Elements of Representative Genotypes of Lonicera edulis",
  annote="The aim of this study was to evaluate the bioactive substances in 19 berry cultivars of edible honeysuckle (Lonicera edulis). A statistical evaluation was used to determine the relationship between the content of selected bioactive substances and individual cultivars. Regarding mineral elements, the content of sodium was measured using potentiometry and spectrophotometry. The content of selected polyphenolic compounds with high antioxidant activity was determined by a HPLC-UV/ED method. The total amount of polyphenols was determined by the Folin-Ciocalteu method. The antioxidant activity was determined using five methods (DPPH, FRAP, ABTS, FR and DMPD) that differ in their principles. The content of 13 amino acids was determined by ion-exchange chromatography. The experimental results obtained for the different cultivars were evaluated and compared by statistical and bioinformatic methods. A unique feature of this study lies in the exhaustive analysis of the chosen parameters (amino acids, mineral elements, polyphenolic compounds and antioxidant activity) during one growing season.",
  address="MDPI",
  chapter="110091",
  doi="10.3390/molecules19056504",
  howpublished="online",
  institution="MDPI",
  number="5",
  volume="19",
  year="2014",
  month="may",
  pages="6505--6523",
  publisher="MDPI",
  type="journal article in Web of Science"
}