Publication detail

Sequential processing of quantitative phase images for the study of cell behaviour in real-time digital holographic microscopy

ZIKMUND, T. KVASNICA, L. TÝČ, M. KŘÍŽOVÁ, A. ČOLLÁKOVÁ, J. CHMELÍK, R.

Original Title

Sequential processing of quantitative phase images for the study of cell behaviour in real-time digital holographic microscopy

Type

journal article in Web of Science

Language

English

Original Abstract

Transmitted light holographic microscopy is particularly used for quantitative phase imaging of transparent microscopic objects such as living cells. The study of the cell is based on extraction of the dynamic data on cell behaviour from the time-lapse sequence of the phase images. However, the phase images are affected by the phase aberrations that make the analysis particularly difficult. This is because the phase deformation is prone to change during long-term experiments. Here, we present a novel algorithm for sequential processing of living cells phase images in a time-lapse sequence. The algorithm compensates for the deformation of a phase image using weighted least-squares surface fitting. Moreover, it identifies and segments the individual cells in the phase image.All these procedures are performed automatically and applied immediately after obtaining every single phase image. This property of the algorithm is important for real-time cell quantitative phase imaging and instantaneous control of the course of the experiment by playback of the recorded sequence up to actual time. Such operators intervention is a forerunner of process automation derived from image analysis. The efficiency of the propounded algorithm is demonstrated on images of rat fibrosarcoma cells using an off-axis holographic microscope.

Keywords

Aberration compensation, cell tracking, digital image processing, quantitative phase imaging, real-time holography.

Authors

ZIKMUND, T.; KVASNICA, L.; TÝČ, M.; KŘÍŽOVÁ, A.; ČOLLÁKOVÁ, J.; CHMELÍK, R.

RIV year

2014

Released

1. 11. 2014

ISBN

0022-2720

Periodical

Journal of Microscopy

Year of study

256

Number

2

State

United Kingdom of Great Britain and Northern Ireland

Pages from

117

Pages to

125

Pages count

9

BibTex

@article{BUT109829,
  author="Tomáš {Zikmund} and Lukáš {Kvasnica} and Matěj {Týč} and Aneta {Křížová} and Jana {Čolláková} and Radim {Chmelík}",
  title="Sequential processing of quantitative phase images for the study of cell behaviour in real-time digital holographic microscopy",
  journal="Journal of Microscopy",
  year="2014",
  volume="256",
  number="2",
  pages="117--125",
  doi="10.1111/jmi.12165",
  issn="0022-2720"
}