Publication detail

Antibiotic susceptibility and resistance of Staphylococcus aureus isolated from fresh porcine skin xenografts: Risk to recipients with thermal injury

BUSBY, S. ROBB, A. LANG, S. TAKEUCHI, Y. VESELÝ, P. SCOBIE, L.

Original Title

Antibiotic susceptibility and resistance of Staphylococcus aureus isolated from fresh porcine skin xenografts: Risk to recipients with thermal injury

Type

journal article in Web of Science

Language

English

Original Abstract

The previous use of fresh porcine xenografts at the Prague Burn Centre had raised concerns over the transmission of zoonotic pathogens. This study examines the risk of zoonotic Staphylococcus aureus colonisation of burn patients from fresh porcine skin xenografts. Samples were collected from the nares, skin and perineum of commercial pigs (n = 101) and were screened for methicillin sensitive S. aureus (MSSA) and resistant S. aureus (MRSA). The efficacy of the antibiotic wash used in decontamination of the pigskin was tested against planktonic- and biofilm-grown isolates. The spa type of each isolate was also confirmed. All pig swabs were negative for MRSA but 86% positive for MSSA. All planktonic-grown isolates of MSSA were sensitive to chloramphenicol and nitrofurantoin and 44% of isolates were resistant to streptomycin. Isolates grown as biofilm exhibited higher rates of antimicrobial resistance. Sequence analysis revealed three distinct spa types of the MRSA ST398 clonal type. This finding demonstrates the existence of a MSSA reservoir containing spa types resembling those of well-known MRSA strains. These MSSA exhibit resistance to antibiotics used for decontamination of the pigskin prior to xenograft. Amended use of procurement could allow the use of fresh pigskin xenografts to be reinstated.

Keywords

Infection, MRSA MSSA Porcine skin

Authors

BUSBY, S.; ROBB, A.; LANG, S.; TAKEUCHI, Y.; VESELÝ, P.; SCOBIE, L.

RIV year

2014

Released

1. 3. 2014

ISBN

0305-4179

Periodical

BURNS

Year of study

40

Number

2

State

United Kingdom of Great Britain and Northern Ireland

Pages from

288

Pages to

294

Pages count

7

BibTex

@article{BUT107929,
  author="Stacey-Ann {Busby} and Andrew {Robb} and Sue {Lang} and Yasuhiro {Takeuchi} and Pavel {Veselý} and Linda {Scobie}",
  title="Antibiotic susceptibility and resistance of Staphylococcus aureus isolated from fresh porcine skin xenografts: Risk to recipients with thermal injury",
  journal="BURNS",
  year="2014",
  volume="40",
  number="2",
  pages="288--294",
  doi="10.1016/j.burns.2013.06.006",
  issn="0305-4179"
}