Publication detail

Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs

KREJČOVÁ, L. HYNEK, D. KOPEL, P. ADAM, V. HUBÁLEK, J. TRNKOVÁ, L. KIZEK, R.

Original Title

Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs

Type

journal article in Web of Science

Language

English

Original Abstract

In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine–adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 degrees Celsius, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 ug mL 1. The detection limit (3 signal/noise) was estimated as 15 ng ml 1 of ODN-SH-Cd.

Keywords

paramagnetic particles, DNA, differential pulse voltammetry, square wave voltammetry

Authors

KREJČOVÁ, L.; HYNEK, D.; KOPEL, P.; ADAM, V.; HUBÁLEK, J.; TRNKOVÁ, L.; KIZEK, R.

RIV year

2013

Released

1. 4. 2013

ISBN

0009-5893

Periodical

CHROMATOGRAPHIA

Year of study

76

Number

7-8

State

Federal Republic of Germany

Pages from

355

Pages to

362

Pages count

8

BibTex

@article{BUT100954,
  author="Ludmila {Krejčová} and David {Hynek} and Pavel {Kopel} and Vojtěch {Adam} and Jaromír {Hubálek} and Libuše {Trnková} and René {Kizek}",
  title="Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs",
  journal="CHROMATOGRAPHIA",
  year="2013",
  volume="76",
  number="7-8",
  pages="355--362",
  doi="10.1007/s10337-012-2327-0",
  issn="0009-5893"
}