Czech

4

Not applicable.

The course Biochemistry II is focused on detailed study of metabolic pathways and their regulation on molecular as well as cellular level. The knowlege obtained after passing the course can serve as basis for further studies, especially for biotechnology, molecular biology and bioengineering.

Examination - Biochemistry I.

Not applicable.

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Credit test. Examination - written and oral part.

1.part – animal cell
- Bioenergetics; mechanisms of enzyme catalysis; regulation of enzyme activity. Cell signalization; regulation of cell metabolism (hormones).
Gene expression and regulation in prokaryotic and eukarytic organisms. Posttranslation modifications.
Primary and secondary metabolism; mteabolic pathways, metabolism of specifc groups of lipids and amino acids. Regulation of intermediary metabolism. Pathobiochemistry.
Secondary metabolites of some animals – feromons, allomons; polyamines.

2.part – microorganisms.
Microorganism types according to energy sources, nutrition and metabolism type. Specificity of microbial metabolism, metabolic adaptation, stress factors, extremophilles.
Primary and secondary metabolism of microorganisms. Fermentation types, regulation of primary metabolite production.
Biosynthesis of selected microbial secondary metabolites - antibiotics, vitamins, polysaccharides..
Biodegradation, bioremediation and biotransformation - overview.

3.part –plants
Plant metabolism, overview of main pathways of primary and secondary metabolism. Photosynthesis.
Production of selected plant secondary metabolites – aromatic compounds, natural colourants, alcaloids, steroids, glykosides, terpenes.

Not applicable.

The aim of the course Biochemistry II is to introduce basic principles of cell metabolism and its regulation. Based on general thermodynamic principles the course of central biochemical pathways including metabolism of saccharides, fatty acids and amino acids is elucidated. Further, basic principles of biological oxidations, cell energy production and photosynthesis are discussed. Next, metabolism of nucleic acids and proteins and processes involved in gene expression are introduced. As a part of course intermediate metabolism and complex regulation of metabolic pathways are presented.

none

Not applicable.

Not applicable.

Vodrážka Z.: Biochemie. Academia Praha, Praha 1999. (CS)
Stryer L.: Biochemistry. New York 1988. (CS)

Alberts B., Bray D., Johnson A., Lewis J., Raff M., Robertson K., Walter P.: Základy buněčné biologie. Espero Publishing, Ústí n. Labem 2005. (CS)
Voet D., Voet J. G.: Biochemie. Victoria Publishing a.s., Praha 1995. (CS)

26 hours, optionally