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| Original Title: | A New Approach how to Define the Coefficient of Electroactivity of Adenine and Its Twelve Derivatives Using Flow Injection Analysis with Amperometric Detection |
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| Czech Title: | Nový přístup, jak definovat koeficient elektroaktivity adeninu a jeho dvanácti derivátů pomocí průtokové injekční analýzy s amperometrickou detekcí. |
| English Title: | A New Approach how to Define the Coefficient of Electroactivity of Adenine and Its Twelve Derivatives Using Flow Injection Analysis with Amperometric Detection |
| Author(s): | ZÍTKA, O.; ŠKUTKOVÁ, H.; ADAM, V.; TRNKOVÁ, L.; BABULA, P.; HUBÁLEK, J.; PROVAZNÍK, I.; KIZEK, R. |
| Type: | journal article |
| Language: | en |
| Original Abstract: | We studied the electrochemical behaviour of adenine derivates (adenosine, 2 - aminopurine, 2,6 - diaminopurine,6 - benzyl - aminopurine, adenosine - monophosphate, cyclic adenosine monophosphate, nicotin amide adenine dinucleotide, adenosine - triphosphate, S-adenosyl - l - methionine and synthetic derivatives AD -3, AD - 6 and AD-9) using flow injection analysis with electrochemical detection. The influences of pH, flow rate and potential on the signal height of the studied derivates were tested. The optimal pH was 3, the flow rate of the mobile phase 0.75 mL min 1 and the potential 1100 mV. Further, we attempted to characterize each of the studied derivatives by mathematical equations and classic analytical parameters.The lowest detecti on limit was estimated for adenine as 0.9 nM and 2 - amino - purine as 0.5nM |
| Czech abstract: | Studovali jsme elektrochemické chování adenin derivátů (adenosin, 2 - aminopurine, 2,6 - diaminopurine, 6 - benzyl - aminopurine, adenosin - monofosfátu, cyklického adenosin monofosfátu, nicotin amid adenin dinukleotid, adenosin - trifosfát, S-adenosyl - l - methionin a syntetické deriváty AD -3, AD - 6 a AD-9) pomocí průtokové injekční analýzy s elektrochemickou detekcí. Dále byly testovány vlivy pH, průtok a potenciál na amplitudu signálu studovaných derivátů. Jako optimální pH byla stanovena hodnota 3, průtok mobilní fáze 0,75 ml min 1 a potenciál 1100 mV. Následně jsme se pokusili charakterizovat každý ze studovaných derivátů matematickými rovnicemi a klasickými analytickými parametery. Nejnižší detekčních na limit byl odhadnut pro adenin 0,9 nM a pro 2- amino-purin jako 0.5nM. |
| English abstract: | We studied the electrochemical behaviour of adenine derivates (adenosine, 2 - aminopurine, 2,6 - diaminopurine,6 - benzyl - aminopurine, adenosine - monophosphate, cyclic adenosine monophosphate, nicotin amide adenine dinucleotide, adenosine - triphosphate, S-adenosyl - l - methionine and synthetic derivatives AD -3, AD - 6 and AD-9) using flow injection analysis with electrochemical detection. The influences of pH, flow rate and potential on the signal height of the studied derivates were tested. The optimal pH was 3, the flow rate of the mobile phase 0.75 mL min 1 and the potential 1100 mV. Further, we attempted to characterize each of the studied derivatives by mathematical equations and classic analytical parameters.The lowest detecti on limit was estimated for adenine as 0.9 nM and 2 - amino - purine as 0.5nM |
| Keywords: | Adenine derivatives, Flow injection analysis, Amperometric detection, Glassy carbon electrode, Electroactivity |
| Released: | 21.06.2011 |
| Publisher: | WILEY |
| Location: | Weinheim |
| Pages from: | 1556 |
| Pages to: | 1567 |
| Pages count: | 12 |
