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Publication detail
| Original Title: | Isoprostanes as markers of oxidative stress |
|---|---|
| Czech Title: | Isoprostany jako markery oxidativního stresu |
| English Title: | Isoprostanes as markers of oxidative stress |
| Author(s): | BOLECHOVÁ, M.; ČÁSLAVSKÝ, J.; VÁVROVÁ, M. |
| Type: | abstract |
| Language: | en |
| Original Abstract: | Oxidative stress is an imbalance between oxidants occurence on organism and its antioxidant defense. Oxidative stress is assessed by determination of secondary products like isoprostanes which is group of prostaglandin-like compounds. These are formed via non enzymatic radical oxidation of polyunsaturated fatty acids with at least four double bonds. One of the most useful method is determination of isoprostanes by HPLC with tandem mass spectrometric detection (HPLC-MS/MS). |
| Czech abstract: | Oxidativní stres je definován jako nerovnováha mezi oxidanty a antioxidativní ochranou v organismu. Oxidativní stres je sledován pomocí sekundárních produktů, tzv. isoprostanů, které patří do skupiny prostaglandinů. Isoprostany jsou formovány neenzymatickou radikálovou oxidací polynenasycených mastných kyselin s nejméně čtyřmi vazbami. Nejpoužívanější metodou pro sledování isoprostanů v organismu je kapalinová chromatografie s tandemovou hmotnostní spektrometrií (HPLC-MS/MS). |
| English abstract: | Oxidative stress is an imbalance between oxidants occurence on organism and its antioxidant defense. Oxidative stress is assessed by determination of secondary products like isoprostanes which is group of prostaglandin-like compounds. These are formed via non enzymatic radical oxidation of polyunsaturated fatty acids with at least four double bonds. One of the most useful method is determination of isoprostanes by HPLC with tandem mass spectrometric detection (HPLC-MS/MS). |
| Keywords: | oxidative stress, fatty acids, isoprostanes, chromatography, mass spectrometry |
| Released: | 17.08.2011 |
| Pages from: | s959 |
| Pages to: | s959 |
| Pages count: | 1 |
